PMID: 6830832Mar 22, 1983

The thromboxane antagonist, 13-azaprostanoic acid, inhibits arachidonic acid-induced Ca2+ release from isolated platelet membrane vesicles

Biochimica Et Biophysica Acta
J P RybickiG C Le Breton


In the present study we investigated the ability of the arachidonic acid metabolites, prostaglandin H2 and thromboxane A2, to release Ca2+ from isolated platelet vesicles. The vesicles were prepared through modification of previously described procedures. 45Ca uptake and release were determined by Millipore filtration and isotope counting of the filter paper. Incubation of the vesicles (25 degrees C) with 50 microM CaCl2 (plus 45Ca) resulted in the accumulation of 13 nmol Ca2+ per mg of protein under steady-state conditions. Addition of arachidonic acid (25 microM) resulted in a 42% release of the accumulated Ca2+ and the production of 150 ng thromboxane B2/mg protein. Pretreatment of the vesicles with indomethacin (4 microM) completely inhibited arachidonic acid-induced Ca2+ release and reduced thromboxane B2 synthesis by 82%. Pretreatment of the vesicles with the specific thromboxane A2/prostaglandin H2 antagonist, 13-azaprostanoic acid (20 microM), also resulted in complete inhibition of Ca2+ release but no inhibition of thromboxane B2 production. Addition of prostaglandin H2 (0.3 microM) to the platelet vesicles produced a significant release of Ca2+ only in the presence of the adenylate cyclase inhibitor, 2',5'-dideoxyaden...Continue Reading


May 1, 1995·Thrombosis Research·T H TsaiC F Chen
Oct 1, 1984·Prostaglandins, Leukotrienes, and Medicine·H L LipptonP J Kadowitz
Oct 13, 2012·The Journal of Biological Chemistry·Adam J WieschhausAthar H Chishti
Jan 22, 1992·Biochemical Pharmacology·S O KimG C Le Breton
Feb 1, 1988·European Journal of Clinical Investigation·K Jaschonek, C P Muller


Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·G A Rodan, M B Feinstein
Jul 1, 1979·Journal of Medicinal Chemistry·D L VentonG C Le Breton
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P NeedlemanM Minkes
Apr 1, 1979·The Journal of Clinical Investigation·S Rittenhouse-Simmons
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G C Le BretonP V Halushka
Jul 12, 1976·Biochemical and Biophysical Research Communications·G C Le BretonH Feinberg
Feb 21, 1977·Biochemical and Biophysical Research Communications·F F Sun
Jan 1, 1980·Annual Review of Biochemistry·R S Adelstein, E Eisenberg
Mar 28, 1980·Biochemical and Biophysical Research Communications·M B Feinstein

Related Concepts

Saturated Fat
Prostaglandin H2
Prostanoic Acids
13-azaprostanoic acid, (trans)-isomer
Eicosatetraenoic Acids
Prostaglandin Endoperoxides, Synthetic
Rabbit Aorta Contracting Substance
In Vitro [Publication Type]

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.