PMID: 6830832Mar 22, 1983

The thromboxane antagonist, 13-azaprostanoic acid, inhibits arachidonic acid-induced Ca2+ release from isolated platelet membrane vesicles

Biochimica Et Biophysica Acta
J P RybickiG C Le Breton

Abstract

In the present study we investigated the ability of the arachidonic acid metabolites, prostaglandin H2 and thromboxane A2, to release Ca2+ from isolated platelet vesicles. The vesicles were prepared through modification of previously described procedures. 45Ca uptake and release were determined by Millipore filtration and isotope counting of the filter paper. Incubation of the vesicles (25 degrees C) with 50 microM CaCl2 (plus 45Ca) resulted in the accumulation of 13 nmol Ca2+ per mg of protein under steady-state conditions. Addition of arachidonic acid (25 microM) resulted in a 42% release of the accumulated Ca2+ and the production of 150 ng thromboxane B2/mg protein. Pretreatment of the vesicles with indomethacin (4 microM) completely inhibited arachidonic acid-induced Ca2+ release and reduced thromboxane B2 synthesis by 82%. Pretreatment of the vesicles with the specific thromboxane A2/prostaglandin H2 antagonist, 13-azaprostanoic acid (20 microM), also resulted in complete inhibition of Ca2+ release but no inhibition of thromboxane B2 production. Addition of prostaglandin H2 (0.3 microM) to the platelet vesicles produced a significant release of Ca2+ only in the presence of the adenylate cyclase inhibitor, 2',5'-dideoxyaden...Continue Reading

References

May 1, 1995·Thrombosis Research·T H TsaiC F Chen
Oct 1, 1984·Prostaglandins, Leukotrienes, and Medicine·H L LipptonP J Kadowitz
Oct 13, 2012·The Journal of Biological Chemistry·Adam J WieschhausAthar H Chishti
Jan 22, 1992·Biochemical Pharmacology·S O KimG C Le Breton
Feb 1, 1988·European Journal of Clinical Investigation·K Jaschonek, C P Muller

Citations

Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·G A Rodan, M B Feinstein
Jul 1, 1979·Journal of Medicinal Chemistry·D L VentonG C Le Breton
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P NeedlemanM Minkes
Apr 1, 1979·The Journal of Clinical Investigation·S Rittenhouse-Simmons
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G C Le BretonP V Halushka
Jul 12, 1976·Biochemical and Biophysical Research Communications·G C Le BretonH Feinberg
Feb 21, 1977·Biochemical and Biophysical Research Communications·F F Sun
Jan 1, 1980·Annual Review of Biochemistry·R S Adelstein, E Eisenberg
Mar 28, 1980·Biochemical and Biophysical Research Communications·M B Feinstein

Related Concepts

Calcium
Saturated Fat
Prostaglandin H2
Prostanoic Acids
13-azaprostanoic acid, (trans)-isomer
Eicosatetraenoic Acids
Striadyne
Prostaglandin Endoperoxides, Synthetic
Rabbit Aorta Contracting Substance
In Vitro [Publication Type]

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