The Tic40 translocon components exhibit preferential interactions with different forms of the Oee1 plastid protein precursor

Functional Plant Biology : FPB
Kenton KoZdenka Ko

Abstract

The transport of proteins across the plastid envelope involves a host of proteinaceous components that attend to varying structural needs of the process. This study focuses on interactions between two select forms (designated Tic40 and Toc36) of the Tic40-related components and different structural versions of the Oee1 precursor to dissect the components' mode of operation. Interaction profiling revealed several features pertaining to how Tic40-related components might work during the transport process. The main operational features revealed are: (1) Tic40 interacts preferentially with Oee1 precursors containing only the plastid-targeting domain, (2) Toc36 interacts preferentially with Oee1 precursors containing both plastid- and thylakoid lumen-targeting domains, (3)carboxyl truncations to either the entire Oee1 precursor or Toc36 affect interactions negatively, and (4) the general reduction of Tic40-related protein levels in transgenic plants appears to exert a greater negative impact on endogenous Oee1 levels than the other proteins assessed, a trend that corroborates the findings of the protein interaction experiments.

Citations

Aug 10, 2005·Journal of Experimental Botany·Jocelyn Bédard, Paul Jarvis
Jan 22, 2005·The Plant Journal : for Cell and Molecular Biology·Sabina KovachevaPaul Jarvis
Oct 30, 2004·The Journal of Biological Chemistry·Kenton KoZdenka Ko
Feb 24, 2021·Protein Science : a Publication of the Protein Society·Michelle Y FryWilliam M Clemons

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