The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron.

Biophysical Journal
Lie-Cheng WangZhuan Zhou

Abstract

Endocytosis is a fundamental cellular event in membrane retrieval after exocytosis and in the regulation of receptor-mediated signal transduction. In contrast to the well-studied depolarization-induced membrane recycling, little is known about the kinetics of ligand-induced endocytosis of G-protein-coupled receptors in neurons. Here we investigated the kinetics of ligand-receptor binding-induced endocytosis in rat sensory neurons using a membrane capacitance assay. The time constant of ADP-induced endocytosis of P2Y-receptors was determined as 1.7 s. The ADP-induced endocytosis was blocked by antagonists against P2Y, phosphorylation, and clathrin. However, block of dynamin was without effect. The ADP-induced endocytosis was confirmed independently by a single vesicle image technique using a styryl FM2-10. Finally, the receptors were internalized in response to ADP, as determined by GFP-labeled P2Y. We conclude that ligand-receptor binding leads to rapid endocytosis in the cytoplasm of rat dorsal root ganglion neurons.

References

Feb 1, 1988·Pflügers Archiv : European journal of physiology·M Lindau, E Neher
May 1, 1973·The Journal of Cell Biology·B CeccarelliA Mauro
Aug 1, 1995·Current Opinion in Cell Biology·M Lindau, W Almers
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·C R ArtalejoH C Palfrey
Jan 6, 1995·The Journal of Biological Chemistry·B KublaouiP F Pilch
Nov 1, 1994·The Journal of Cell Biology·H DamkeS L Schmid
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·T A RyanH Reuter
Jul 15, 1996·The Journal of Physiology·S F Hsu, M B Jackson
Dec 31, 1997·The Journal of Cell Biology·C Smith, E Neher
Apr 29, 1998·Annual Review of Physiology·W J Betz, J K Angleson
May 20, 1999·Current Biology : CB·H T McMahon
Apr 18, 2001·Trends in Cell Biology·G D Holman, I V Sandoval
Dec 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·G NevesL Lagnado
Jan 24, 2002·Nature·Howard A RockmanRobert J Lefkowitz
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Cristina R ArtalejoH Clive Palfrey
May 30, 2002·The Journal of Biological Chemistry·Zhongxian LuYe-Guang Chen
Dec 6, 2002·Pflügers Archiv : European journal of physiology·Helena H ChowdhuryRobert Zorec
Mar 7, 2003·Nature·Sean D Conner, Sandra L Schmid
May 16, 2003·Current Biology : CB·H Clive Palfrey, Cristina R Artalejo
Jun 6, 2003·Nature·Sunil P Gandhi, Charles F Stevens
Mar 26, 2004·Nature Reviews. Molecular Cell Biology·Frederick R Maxfield, Timothy E McGraw
Dec 1, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Bei WuZhuan Zhou
Sep 13, 2005·Annals of the New York Academy of Sciences·Helena H ChowdhuryRobert Zorec

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Citations

Dec 4, 2015·Toxins·Masataka OdaMasahiro Nagahama
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
Aug 2, 2013·American Journal of Physiology. Cell Physiology·Liang-Hong ZhengZhuan Zhou

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