The Timing of Midzone Stabilization during Cytokinesis Depends on Myosin II Activity and an Interaction between INCENP and Actin

Current Biology : CB
Jennifer Landino, Ryoma Ohi

Abstract

The final steps of cell division are tightly coordinated in space and time, but whether mechanisms exist to couple the actin and microtubule (MT) cytoskeletons during anaphase and cytokinesis (C phase) is largely unknown. During anaphase, MTs are incorporated into an anti-parallel array termed the spindle midzone (midzone MTs), whereas F-actin and non-muscle myosin II, together with other factors, organize into the cleavage furrow [1]. Previous studies in somatic cells have shown that midzone MTs become highly stable after furrows have begun ingression [2], indicating that furrow-to-MT communication may occur. Midzone formation is also inhibited in fly spermatocytes that fail to form a cleavage furrow [3] and during monopolar cytokinesis when myosin contractility is blocked by blebbistatin [4]. We show here that midzone MT stabilization is dependent on actomyosin contraction, suggesting that there is active coordination between furrow ingression and microtubule dynamics. Midzone microtubule stabilization also depends on the kinase activity of Aurora B, the catalytic subunit of the chromosomal passenger complex (CPC), uncovering a feedback mechanism that couples furrowing with microtubule dynamics. We further show that the CPC s...Continue Reading

References

Mar 15, 2003·Science·Aaron F StraightTimothy J Mitchison
Apr 30, 2003·The Journal of Cell Biology·Claire DitchfieldStephen S Taylor
Aug 9, 2003·Nature·Julie C CanmanE D Salmon
Jan 12, 2005·Biochemistry·Takeshi SakamotoJames R Sellers
Apr 16, 2008·The Journal of Cell Biology·Chi-Kuo HuTimothy J Mitchison
May 14, 2011·Current Biology : CB·Chi-Kuo HuTimothy J Mitchison
Apr 18, 2012·Biochimica Et Biophysica Acta·Aliaksandr MikulichPetras Juzenas
Jul 19, 2012·Annual Review of Cell and Developmental Biology·Rebecca A GreenKaren Oegema
Nov 24, 2012·Nature Reviews. Molecular Cell Biology·Mar CarmenaWilliam C Earnshaw
Jun 24, 2014·Angewandte Chemie·Miklós KépiróAndrás Málnási-Csizmadia
Oct 11, 2014·Science·Phuong A NguyenTimothy J Mitchison

❮ Previous
Next ❯

Citations

Aug 26, 2016·Molecular Biology of the Cell·Megan M GnazzoAhna R Skop
Mar 30, 2017·Frontiers in Molecular Neuroscience·Yan WangJunping Kou
Aug 16, 2018·Science Signaling·Vladimir Joukov, Arcangela De Nicolo
May 9, 2018·Current Biology : CB·Angika Basant, Michael Glotzer
Sep 4, 2019·Developmental Biology·Leslie Toledo-JacoboCharles B Shuster
May 28, 2021·Journal of Cell Science·Patricia Wadsworth
Aug 4, 2021·The Journal of Biological Chemistry·Aryeh BabkoffShoshana Ravid
Jan 8, 2022·The Journal of Cell Biology·Sophia M HirschJulie C Canman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.