The timing of protein kinase activation events in the cascade that regulates mitotic progression in Tradescantia stamen hair cells

The Plant Cell
S M Wolniak, P M Larsen

Abstract

Stamen hair cells of the spiderwort plant Tradescantia virginiana exhibit unusually predictable rates of progression through mitosis, particularly from the time of nuclear envelope breakdown (NEBD) through the initiation of cytokinesis. The predictable rate of progression through prometaphase and metaphase has made these cells a useful model system for the determination of the timing of regulatory events that trigger entry into anaphase. A number of studies suggest that the elevation of one or more protein kinase activities is a necessary prerequisite for entry into anaphase. The current experiments employ two strategies to test when these elevations in protein kinase activity actually occur during metaphase. In perfusions, we added the protein kinase inhibitors K-252a, staurosporine, or calphostin C to living stamen hair cells for 10-min intervals at known times during prometaphase or metaphase and monitored the subsequent rate of progression into anaphase. Metaphase transit times were altered as a function of the time of addition of K-252a or staurosporine to the cells; metaphase transit times were extended significantly by treatments initiated in prometaphase through early metaphase and again late in metaphase. Transit times...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·C Norbury, P Nurse
May 1, 1991·The Plant Cell·P C FerreiraD Inzé
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J ColasantiV Sundaresan
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H S Feiler, T W Jacobs
Dec 31, 1990·Biochemical and Biophysical Research Communications·K OhmiY Nonomura
Aug 31, 1990·Biochemical and Biophysical Research Communications·J M HerbertJ P Maffrand
Jan 1, 1988·Annual Review of Cell Biology·L Gerace, B Burke
Dec 18, 1989·FEBS Letters·P D DavisS E Wilkinson
Jul 28, 1989·Biochimica Et Biophysica Acta·C J Norbury, P Nurse
Jul 28, 1989·Cell·S MorenoP Nurse
Dec 1, 1989·The Journal of Cell Biology·P K Hepler
Jun 1, 1988·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·S M Wolniak
Nov 1, 1994·Trends in Biochemical Sciences·C MacKintosh, R W MacKintosh
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C LeeA M Edelman
Nov 30, 1993·Biochemical and Biophysical Research Communications·L MorelloD Breviario

❮ Previous
Next ❯

Citations

Aug 14, 1999·International Journal of Cancer. Journal International Du Cancer·A M ParissentiS Glück
Apr 24, 1999·The Plant Cell·A E Franklin, W Z Cande
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Desh Pal S Verma
Aug 5, 2005·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Zhuo ZhangHui Wang
Dec 25, 2009·The Plant Journal : for Cell and Molecular Biology·Hao WangLiwen Jiang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.