The Tlo proteins are stoichiometric components of Candida albicans mediator anchored via the Med3 subunit.

Eukaryotic Cell
Anda ZhangLawrence C Myers

Abstract

The amplification of the TLO (for telomere-associated) genes in Candida albicans, compared to its less pathogenic, close relative Candida dubliniensis, suggests a role in virulence. Little, however, is known about the function of the Tlo proteins. We have purified the Mediator coactivator complex from C. albicans (caMediator) and found that Tlo proteins are a stoichiometric component of caMediator. Many members of the Tlo family are expressed, and each is a unique member of caMediator. Protein expression analysis of individual Tlo proteins, as well as the purification of tagged Tlo proteins, demonstrate that there is a large free population of Tlo proteins in addition to the Mediator-associated population. Coexpression and copurification of Tloα12 and caMed3 in Escherichia coli established a direct physical interaction between the two proteins. We have also made a C. albicans med3Δ/Δ strain and purified an intact Mediator from this strain. The analysis of the composition of the med3Δ Mediator shows that it lacks a Tlo subunit. Regarding Mediator function, the med3Δ/Δ strain serves as a substitute for the difficult-to-make tloΔ/Δ C. albicans strain. A potential role of the TLO and MED3 genes in virulence is supported by the inab...Continue Reading

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Citations

Oct 2, 2015·Briefings in Functional Genomics·Matthew Z Anderson, Richard J Bennett
Jun 9, 2015·Trends in Genetics : TIG·Malcolm WhitewayRoberto Rodríguez-Ortiz
Nov 7, 2014·PLoS Genetics·Faiza TebbjiMalcolm Whiteway
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Nov 2, 2016·Nucleic Acids Research·Verónica Freire-BenéitezAlessia Buscaino
May 20, 2018·Applied and Environmental Microbiology·Hui LiuLiming Liu
Mar 10, 2015·Eukaryotic Cell·S D WillgerD A Hogan
Aug 16, 2017·Antimicrobial Agents and Chemotherapy·Zhongle Liu, Lawrence C Myers
Jun 21, 2017·The Journal of Cell Biology·Sierra N CullatiScott A Gerber
Jan 15, 2019·Current Genetics·Gary P MoranDerek J Sullivan
Jul 22, 2018·PloS One·Peter R FlanaganDerek J Sullivan
Aug 24, 2019·Frontiers in Microbiology·Floris Schoeters, Patrick Van Dijck
Mar 18, 2016·Oncology Reports·Emilie Laure DuboisRegina Groisman
May 10, 2015·Genetics·Matthew Z AndersonJudith Berman
Mar 4, 2019·Trends in Genetics : TIG·Mélanie LegrandKaustuv Sanyal
Jul 29, 2017·Microbiology Spectrum·Allyson A ErlendsonMichael Freitag
Dec 17, 2014·Genome Research·Matthew P HirakawaChristina A Cuomo

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