PMID: 9171417Jun 1, 1997Paper

The TolA protein interacts with colicin E1 differently than with other group A colicins

Journal of Bacteriology
S L SchendelWilliam A Cramer

Abstract

The 421-residue protein TolA is required for the translocation of group A colicins (colicins E1, E2, E3, A, K, and N) across the cell envelope of Escherichia coli. Mutations in TolA can render cells tolerant to these colicins and cause hypersensitivity to detergents and certain antibiotics, as well as a tendency to leak periplasmic proteins. TolA contains a long alpha-helical domain which connects a membrane anchor to the C-terminal domain, which is required for colicin sensitivity. The functional role of the alpha-helical domain was tested by deletion of residues 56 to 169 (TolA delta1), 166 to 287 (TolA delta2), or 54 to 287 (TolA delta3) of the alpha-helical domain of TolA, which removed the N-terminal half, the C-terminal half, or nearly the entire alpha-helical domain of TolA, respectively. TolA and TolA deletion mutants were expressed from a plasmid in an E. coli strain producing no chromosomally encoded TolA. Cellular sensitivity to the detergent deoxycholate was increased for each deletion mutant, implying that more than half of the TolA alpha-helical domain is necessary for cell envelope stability. Removal of either the N- or C-terminal half of the alpha-helical domain resulted in a slight (ca. 5-fold) decrease in cyto...Continue Reading

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Citations

Sep 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gail E FanucciDavid S Cafiso
Nov 28, 2012·Biochemical Society Transactions·Stanislav D ZakharovWilliam A Cramer
Sep 1, 2012·Annual Review of Genetics·Karen S Jakes, William A Cramer
Jan 24, 2014·Nucleic Acids Research·Christopher J GreggGeorge M Church
Aug 1, 1999·Evolution; International Journal of Organic Evolution·Michael Feldgarden, Margaret A Riley
Dec 14, 2018·The Biochemical Journal·William A CramerS D Zakharov
Apr 8, 2003·The Journal of Biological Chemistry·Gregor AnderluhJeremy H Lakey
Nov 28, 2012·Biochemical Society Transactions·Christopher N PenfoldRichard James
Mar 9, 2004·The Journal of Biological Chemistry·Gregor AnderluhJeremy H Lakey
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Feb 6, 2007·Journal of Bacteriology·Muriel MasiRajeev Misra
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
May 31, 2020·FEMS Microbiology Reviews·Joanna SzczepaniakColin Kleanthous
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Nov 25, 1998·Research in Microbiology·R el KouhenJ M Pagès
Nov 1, 2002·Biochimica Et Biophysica Acta·Stanislav D Zakharov, William A Cramer
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Aug 19, 2016·Biochemistry·Stanislav D ZakharovWilliam A Cramer
Oct 14, 2008·BMC Genomics·Chantal W NdeWilliam E Bentley
Jul 29, 2000·Biochemistry·L G DoverJ H Lakey

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