The tomato Hsf system: HsfA2 needs interaction with HsfA1 for efficient nuclear import and may be localized in cytoplasmic heat stress granules.

Molecular and Cellular Biology
Klaus-Dieter ScharfL Nover

Abstract

In heat-stressed (HS) tomato (Lycopersicon peruvianum) cell cultures, the constitutively expressed HS transcription factor HsfA1 is complemented by two HS-inducible forms, HsfA2 and HsfB1. Because of its stability, HsfA2 accumulates to fairly high levels in the course of a prolonged HS and recovery regimen. Using immunofluorescence and cell fractionation experiments, we identified three states of HsfA2: (i) a soluble, cytoplasmic form in preinduced cultures maintained at 25 degrees C, (ii) a salt-resistant, nuclear form found in HS cells, and (iii) a stored form of HsfA2 in cytoplasmic HS granules. The efficient nuclear transport of HsfA2 evidently requires interaction with HsfA1. When expressed in tobacco protoplasts by use of a transient-expression system, HsfA2 is mainly retained in the cytoplasm unless it is coexpressed with HsfA1. The essential parts for the interaction and nuclear cotransport of the two Hsfs are the homologous oligomerization domain (HR-A/B region of the A-type Hsfs) and functional nuclear localization signal motifs of both partners. Direct physical interaction of the two Hsfs with formation of relatively stabile hetero-oligomers was shown by a two-hybrid test in Saccharomyces cerevisiae as well as by coi...Continue Reading

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Citations

Apr 23, 2013·Plant Science : an International Journal of Experimental Plant Biology·Jana PiterkováMarek Petřivalský
Oct 29, 2011·Biochimica Et Biophysica Acta·Klaus-Dieter ScharfLutz Nover
Apr 15, 2008·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jingkang GuoJian Wang
Sep 11, 2007·Trends in Plant Science·Pascal von Koskull-DöringLutz Nover
Apr 16, 2009·The Plant Journal : for Cell and Molecular Biology·David Meiri, Adina Breiman
Oct 25, 2006·The Plant Journal : for Cell and Molecular Biology·Ayako NishizawaShigeru Shigeoka
Jan 22, 2009·Plant, Cell & Environment·Joanna TrippKlaus-Dieter Scharf
Jul 23, 2008·The Plant Journal : for Cell and Molecular Biology·Christian WeberMarkus Fauth
May 7, 2010·Plant, Cell & Environment·Javier Tejedor-CanoJuan Jordano
Aug 16, 2014·Plant, Cell & Environment·Sotirios FragkostefanakisEnrico Schleiff
Apr 12, 2016·Frontiers in Plant Science·Ai-Zhen Sun, Fang-Qing Guo
Sep 1, 2006·Plant Science : an International Journal of Experimental Plant Biology·Jeremy N FriedbergEva Czarnecka-Verner
Oct 18, 2011·Biochimica Et Biophysica Acta·Yasuhiro Kadota, Ken Shirasu
Apr 21, 2016·Bioscience, Biotechnology, and Biochemistry·Yukinori Yabuta
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Dec 1, 2010·Proceedings of the National Academy of Sciences of the United States of America·Raúl CarrancoJuan Jordano
Dec 31, 2004·Journal of Biosciences·Sanjeev Kumar BaniwalPascal von Koskull-Döring
Dec 18, 2004·Plant Molecular Biology·Eva Czarnecka-VernerWilliam B Gurley

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