The toxic effects of microcystin-LR on rat spermatogonia in vitro

Toxicology Letters
Yuan ZhouXiaodong Han

Abstract

Microcystin-leucine arginine (MC-LR), a cyclic heptapeptide produced by several bloom-forming cyanobacteria, has strong reproductive toxicity. We examined whether MC-LR could enter spermatogonia and investigated the toxic effects of MC-LR on spermatogonia in vitro. Multispecific organic anion-transporting polypeptides (Oatps), which transported MCs, were screened as well. Spermatogonia were exposed to 0, 0.5, 5, 50, and 500 nmol/L (nM) MC-LR for 6 h. Cell viability and total antioxidant capacity significantly decreased, meanwhile, the ratio of apoptotic cells, reactive oxidative species (ROS) production, mitochondrial membrane potential (MMP), and intracellular free Ca²⁺ increased after exposure to 5 nM and higher concentrations of MC-LR. MC-LR can immigrate into spermatogonia. At least 5 Oatps (Oatp1a5, -3a1, -6b1, -6c1, and -6d1) were detected at the mRNA level in spermatogonia, and the expression of these Oatps was affected by MC-LR, especially Oatp3a1. This study demonstrated that MC-LR can be transported into spermatogonia and leads to cytotoxicity.

References

Jun 11, 1990·Biochimica Et Biophysica Acta·J E ErikssonJ A Meriluoto
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·M F WatanabeM Suzuki
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M CraigC F Holmes
Oct 21, 1999·Toxicologic Pathology·R E Guzman, P F Solter
Aug 2, 2001·Environmental Science & Technology·P SohoniJ P Sumpter
Aug 2, 2001·Biochemical and Biophysical Research Communications·W X DingC N Ong
Jul 2, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·F IzadyarD G de Rooij
Mar 24, 2004·Toxicon : Official Journal of the International Society on Toxinology·Nicolette BothaEnid G Shephard
May 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yunhan HongJianfang Gui
Mar 2, 2005·Toxicology and Applied Pharmacology·W J FischerB Hagenbuch
Feb 7, 2006·Toxicon : Official Journal of the International Society on Toxinology·Paulina SicińskaWirgiliusz Duda
Aug 26, 2006·Nature Clinical Practice. Endocrinology & Metabolism·Hiroshi Kubota, Ralph L Brinster
Mar 21, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Masaharu KomatsuToru Takeuchi
May 15, 2007·Environmental Toxicology·Cayelan C CareyKathryn L Cottingham
May 25, 2007·Toxicon : Official Journal of the International Society on Toxinology·Raquel M SoaresSandra M F O Azevedo
Aug 21, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S PichardoG Repetto
Oct 27, 2007·Free Radical Research·Qingqing NongToru Takeuchi
Dec 21, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ming DaiHehua Lei
Nov 26, 2008·Toxicology and Applied Pharmacology·D FeursteinD R Dietrich
Aug 14, 2009·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Zongyuan LiShuxian Qu
Jan 28, 2010·Pharmacological Reviews·Curtis D Klaassen, Lauren M Aleksunes
Feb 18, 2010·International Journal of Molecular Sciences·Alexandre Campos, Vitor Vasconcelos

❮ Previous
Next ❯

Citations

Jul 31, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xueting WangXiaodong Han
Jun 20, 2015·Toxins·Zhu ZhuGuoqing Shi
Mar 1, 2016·Journal of Toxicology·Yaqoob LoneRaj Kumar Koiri
Dec 5, 2015·Frontiers in Microbiology·Rajesh P RastogiAran Incharoensakdi
Sep 19, 2015·Environmental Science & Technology·Ondrej AdamovskyLudek Blaha
Nov 2, 2015·Journal of Hazardous Materials·Liang ChenPing Xie
Sep 21, 2013·Toxicon : Official Journal of the International Society on Toxinology·Yu ChenXiaodong Han
Oct 21, 2016·Environmental Toxicology·Yuan ZhouGuang Yu
Nov 5, 2016·Toxicon : Official Journal of the International Society on Toxinology·Xueting WangXiaodong Han
Oct 19, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ling ZhangXiaodong Han
Dec 3, 2014·Molecular and Cellular Biochemistry·Yinghong JiYi Lu
Apr 24, 2017·Toxicology Research·Nicole L McLellan, Richard A Manderville
Feb 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kai DongYalin Dong
May 1, 2014·Spermatogenesis·David M Klein, Nathan J Cherrington
Sep 30, 2020·Environmental Research·Shiyu ZhangHuizhen Zhang
Jan 11, 2019·Toxicon : Official Journal of the International Society on Toxinology·Qin QiaoBenjamin Marie
May 8, 2014·Environmental Science & Technology·Yuan ZhouXiaodong Han

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.