The toxic effects of polychlorinated biphenyl (Aroclor 1242) on Tm3 Leydig cells

Toxicology and Industrial Health
Yasemin Aydin, Melike Erkan

Abstract

Polychlorinated biphenyls (PCBs) are ubiquitous and persistent environmental contaminants that disrupt endocrine function in biological systems, especially in the male reproductive system. Previous studies on the reproductive toxicity of PCBs have focused on the impairment of spermatogenesis, disruption of steroidogenesis, decreased sperm number, and infertility. Aroclor 1242 is a commercial mixture with an average of 42% chlorine by weight. The purpose of the present study was to elucidate the hazardous effects of Aroclor 1242 on Leydig cells through an evaluation of cell viability, lipid peroxidation, hydroxyl radicals, H2O2 production, antioxidant enzymes, and steroidogenic enzymes. Leydig cells were exposed to Aroclor 1242 for 24 h under basal and luteinizing hormone-stimulated conditions at different concentrations (ranging from 10-16 M to 10-6 M). After incubation, Leydig cells were measured for cell viability, lipid peroxidation, reactive oxygen species (hydroxyl radical and H2O2), antioxidant enzymes (catalase, superoxide dismutase, glutathione peroxidase, and glutathione-S-transferase), and steroidogenic enzymes (3β-hydroxysteroid dehydrogenase [HSD] and 17β-HSD). The results showed that cell viability was reduced only...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·B Halliwell, J M Gutteridge
Jun 1, 1987·Bulletin of Environmental Contamination and Toxicology·D B SagerD Girard
May 29, 1987·Biochemical and Biophysical Research Communications·T P Devasagayam, U Tarachand
Apr 1, 1974·Toxicology and Applied Pharmacology·G J JohnstoneO Hutzinger
Jun 1, 1972·Analytical Biochemistry·A K Sinha
May 1, 1974·The Journal of Nutrition·D G HafemanW G Hoekstra
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·R KovacevićS Andrić
May 20, 2000·Biology of Reproduction·S A AndricR Z Kovacevic
Sep 28, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·H LilienthalG Winneke
Nov 23, 2000·Nature·T Finkel, N J Holbrook
Aug 16, 2003·Toxicology Letters·P ApostoliM Manno
Sep 19, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sivanantham BanudeviJagadeesan Arunakaran
Feb 7, 2007·The Journal of Endocrinology·Palaniappan MurugesanJagadeesan Arunakaran
Jun 17, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Prabhu VenkataramanJagadeesan Arunakaran
Mar 13, 2009·Trends in Endocrinology and Metabolism : TEM·Guo-Xin HuXiao-Kun Li
Mar 25, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hamdy A A AlyAshraf B Abdel-Naim
Jun 9, 2009·Endocrine Reviews·Evanthia Diamanti-KandarakisAndrea C Gore
Sep 24, 2010·Journal of Biomedicine & Biotechnology·K SvechnikovO Söder
Dec 6, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Leping YeRen-Shan Ge
May 8, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jiali CaiZhenghong Zuo
May 21, 2013·Environmental Toxicology and Pharmacology·Hamdy A A Aly

❮ Previous
Next ❯

Citations

May 19, 2018·Current Urology Reports·Mahmoud MimaSamuel Ohlander
May 25, 2020·Andrologia·Vaithinathan SelvarajuRalf Henkel
Sep 12, 2018·International Journal of Environmental Research and Public Health·Maria Skaalum PetersenPál Weihe
Dec 2, 2020·Ecotoxicology and Environmental Safety·Yongqi LiYuefeng Cai

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
MDA

Software Mentioned

GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.