The toxicity of multi-walled carbon nanotubes (MWCNTs) to human endothelial cells: The influence of diameters of MWCNTs

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Xuqi ZhaoYi Cao

Abstract

The biological applications of multi-walled carbon nanotubes (MWCNTs) may lead to their exposure to human blood vessels, but the influence of their physicochemical properties on toxicity to endothelial cells is incompletely known. Here, human umbilical vein endothelial cells (HUVECs) were exposed to three commercially available MWCNTs, namely XFM4, XFM22, and XFM34 (diameters XFM4 < XFM22 < XFM34), to understand the possible role of their diameter on toxicity. Based on the same mass concentration, XFM4 induced significantly higher level of cytotoxicity than the other two MWCNTs, and HUVECs internalized more XFM4. Cytokine release, monocyte adhesion, and intracellular reactive oxygen species levels were significantly induced only after XFM4 treatment. The exposure to XFM4 significantly reduced the expression of autophagic genes autophagy-related 7 (ATG7), autophagy-related 12 (ATG12), and beclin 1 (BECN1) and increased the expression of endoplasmic reticulum (ER) stress genes DNA damage inducible transcript 3 (DDIT3) and X-box binding protein 1 spliced (XBP-1s). Moreover, the modulation of autophagy-ER stress by chemicals resulted in a significant increase in the cytotoxicity of XFM4 but had minimal impact on the cytotoxicity of...Continue Reading

Citations

Feb 9, 2020·Nanomaterials·Kaoru Aoki, Naoto Saito
Nov 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Amjad Ali KhanArshad Husain Rahmani
May 26, 2021·The Science of the Total Environment·Abraão Tiago Batista Guimarães, Guilherme Malafaia
Nov 7, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yanli ZhangLongquan Shao

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