PMID: 12770132May 29, 2003Paper

The toxin Tx4(6-1) from the spider Phoneutria nigriventer slows down Na(+) current inactivation in insect CNS via binding to receptor site 3

Journal of Insect Physiology
Maria Elena de LimaM Pelhate

Abstract

Tx4(6-1) a neurotoxic peptide from the venom of the aggressive South American 'armed' spider Phoneutria nigriventer, has been previously isolated and sequenced. It shows no detectable activity in mice but affects the peripheral nervous system of insects by stimulating glutamate release at the neuromuscular junction. Here we investigate possible interactions of the toxin with voltage-activated sodium channels (Na(v)). We confirm that it is ineffective on mammalian Na(v) channels, and establish that it competes with the alpha-like toxin 125I-Bom IV, for binding on the site 3 of insect Na(v) channel (IC(50) value around 25nM). The physiological consequences of this binding to the insect Na(v) channel are shown by electrophysiology: Tx4(6-1) prolongs evoked axonal action potentials (APs) (<500&mgr;s duration in control). Prolonged 8-10ms or 'plateau' 500-800ms APs accompanied by repetitive firing at 80-150Hz are recorded after 4-8min of toxin action. This modification of evoked activity is due to a slowing down of sodium current inactivation. Effects of Tx4(6-1) on sodium current are compared with those of a typical scorpion alpha-toxin and of some other spider toxins active on insect Na(v) channels. At the end of long voltage puls...Continue Reading

References

Oct 1, 1977·Analytical Biochemistry·H RochatS Lissitzky
Mar 1, 1976·The Journal of General Physiology·B Hille, D T Campbell
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R LlinásB Cherksey
Jan 1, 1989·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·M E AdamsV J Venema
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Jan 1, 1981·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·T Piek
Jan 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·S G FigueiredoM Richardson
Oct 1, 1994·Pflügers Archiv : European journal of physiology·G M NicholsonT Narahashi
Feb 1, 1993·Naunyn-Schmiedeberg's Archives of Pharmacology·D A AraújoP S Beirão
Nov 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·G M NicholsonT Narahashi
Nov 22, 2000·Biochimie·S Cestèle, W A Catterall
Nov 22, 2000·Biochimie·P EscoubasG Corzo
Feb 27, 2001·Toxicon : Official Journal of the International Society on Toxinology·C KushmerickM A Prado

❮ Previous
Next ❯

Citations

Mar 10, 2010·Biochemistry. Biokhimii︠a︡·A A VassilevskiE V Grishin
Mar 26, 2013·Cellular and Molecular Life Sciences : CMLS·Jennifer J SmithPaul F Alewood
Jul 2, 2005·FEBS Letters·Enrico LeipoldStefan H Heinemann
Jul 9, 2008·Toxicon : Official Journal of the International Society on Toxinology·Elba VillegasGerardo Corzo
Jan 16, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·M E De LimaM Pelhate
Nov 30, 2012·Biopolymers·Elisabeth F SchwartzMárcia R Mortari
Jul 30, 2015·Toxicon : Official Journal of the International Society on Toxinology·Leida Calegário de OliveiraMaria Elena De Lima
May 28, 2010·Journal of Zhejiang University. Science. B·Rui-lan WangSong-ping Liang
Jan 16, 2007·Toxicon : Official Journal of the International Society on Toxinology·Graham M Nicholson
Jun 29, 2012·Toxins·Monique J WindleyGraham M Nicholson
Aug 31, 2019·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Camila Franco Batista OliveiraMaria Elena de Lima
Nov 10, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·M RichardsonM N Cordeiro
Mar 25, 2019·Toxicon : Official Journal of the International Society on Toxinology·Ana L B PaivaMarcelo R V Diniz
Feb 6, 2004·Toxicon : Official Journal of the International Society on Toxinology·Leida Calegário OliveiraSuely G Figueiredo
Jul 16, 2020·Toxicon : Official Journal of the International Society on Toxinology·Pedro Santana Sales LauriaLuciana Lyra Casais-E-Silva

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.