The Toxoplasma gondii dense granule protein TgGRA3 interacts with host Golgi and dysregulates anterograde transport

Biology Open
Maika S DeffieuStanislas Tomavo

Abstract

After entry into the host cell, the intracellular parasite Toxoplasma gondii resides within a membrane-bound compartment, the parasitophorous vacuole (PV). The PV defines an intracellular, parasite-specific niche surrounded by host organelles, including the Golgi apparatus. The mechanism by which T. gondii hijacks the host Golgi and subverts its functions remains unknown. Here, we present evidence that the dense granule protein TgGRA3 interacts with host Golgi, leading to formation of tubules and entry of host Golgi material into the PV. Targeted disruption of the TgGRA3 gene delays this engulfment of host Golgi. We also demonstrate that TgGRA3 oligomerizes and binds directly to host Golgi membranes. In addition, we show that TgGRA3 dysregulates anterograde transport in the host cell, thereby revealing one of mechanisms employed by T. gondii to recruit host organelles and divert their functions.

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Citations

Jul 19, 2020·Annual Review of Microbiology·Yifan WangJeroen P J Saeij
May 21, 2021·Journal of Cell Science·Mariana De NizCarolina Agop-Nersesian

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Methods Mentioned

BETA
GTPases
two-hybrid
transgenic
confocal microscopy
PCR
Size-exclusion chromatography
gel filtration
electrophoresis
immunoprecipitation
transfection

Software Mentioned

Imaris XT
Proteome Discoverer
GraphPad
HHpred
SOPMA
SWISS
GraphPad Prism
COILS
MODEL
HeliQuest

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