The TraA relaxase autoregulates the putative type IV secretion-like system encoded by the broad-host-range Streptococcus agalactiae plasmid pIP501

Microbiology
Brigitta KurenbachElisabeth Grohmann

Abstract

The conjugative multiple antibiotic resistance plasmid pIP501 can be transferred and stably maintained in a variety of Gram-positive genera, including multicellular Streptomyces lividans, as well as in Gram-negative Escherichia coli. The 15 putative pIP501 transfer (tra) genes are organized in an operon-like structure terminating in a strong transcriptional terminator. This paper reports co-transcription of the pIP501 tra genes in exponentially growing Enterococcus faecalis JH2-2 cells, as shown by RT-PCR. The tra genes are expressed throughout the life cycle of Ent. faecalis, and the expression level is independent of the growth phase. Electrophoretic mobility shift assays indicated that the TraA relaxase, the first gene of the tra operon, binds to the tra promoter P(tra), which partially overlaps with the origin of transfer (oriT). DNase I footprinting experiments further delimited the TraA binding region and defined the nucleotides bound by TraA. Beta-Galactosidase assays with P(tra)-lacZ fusions proved P(tra) promoter activity, which was strongly repressed when TraA was supplied in trans. Thus, it is concluded that the pIP501 tra operon is negatively autoregulated at the transcriptional level by the conjugative DNA relaxase...Continue Reading

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Citations

Jun 19, 2013·Veterinary Microbiology·Ashley N HainesWolfgang K Vogelbein
Nov 29, 2012·The Journal of Biological Chemistry·Nikolaus Goessweiner-MohrWalter Keller
Feb 8, 2011·Nucleic Acids Research·Fabián Lorenzo-DíazManuel Espinosa
Feb 7, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Nikolaus Goessweiner-MohrWalter Keller
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Cristina E Alvarez-Martinez, Peter J Christie
Jul 17, 2010·Future Microbiology·Laura S Frost, Günther Koraimann
Oct 17, 2013·Plasmid·Nikolaus Goessweiner-MohrElisabeth Grohmann
Sep 10, 2014·Acta Crystallographica. Section D, Biological Crystallography·Nikolaus Goessweiner-MohrWalter Keller
Apr 5, 2014·Acta Crystallographica. Section D, Biological Crystallography·Nikolaus Goessweiner-MohrWalter Keller
May 28, 2011·International Journal of Food Microbiology·Masataka SatomiYutaka Yano
Sep 2, 2008·Plasmid·Géraldine Van der Auwera, Jacques Mahillon
Nov 13, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Nikolaus Goessweiner-MohrWalter Keller
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Jun 23, 2018·Plasmid·Verena KohlerElisabeth Grohmann
Jun 25, 2015·Microbiology Spectrum·Nikolaus Goessweiner-MohrElisabeth Grohmann

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