The transcription activation domain of Rpn4 is separate from its degrons

The International Journal of Biochemistry & Cell Biology
Donghong JuYouming Xie

Abstract

The proteasome homeostasis in Saccharomyces cerevisiae is regulated by a negative feedback circuit in which the transcription activator Rpn4 upregulates the proteasome genes and is rapidly degraded by the assembled proteasome. In addition to the proteasome genes, Rpn4 regulates numerous other genes involved in a wide variety of cellular processes. However, the transcription activation domain of Rpn4 remains largely unclear. Here we locate a major transactivation domain of Rpn4 in the N-terminal region between residues 118 and 210. Interestingly, this domain is separate from its degradation signals (degrons), suggesting that functional overlap of sequences that activate transcription and signal degradation may not be as common as previously thought. We further demonstrate that the intracellular proteasome activity is correlated with the transactivation potency of Rpn4. This study provides important information for further understanding the biological functions of Rpn4 and the proteasome system.

References

May 25, 1990·Nucleic Acids Research·M E SchmittB L Trumpower
Jul 21, 1995·The Journal of Biological Chemistry·E S JohnsonA Varshavsky
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y Xie, A Varshavsky
Dec 12, 2001·The Journal of Biological Chemistry·Cezary Wójcik, George N DeMartino
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Masafumi Muratani, William P Tansey
Oct 3, 2003·Nature Cell Biology·J Russell Lipford, Raymond J Deshaies
Apr 20, 2004·The Journal of Biological Chemistry·Donghong Ju, Youming Xie
Jun 5, 2004·FEBS Letters·Markus K LondonR Jürgen Dohmen
Sep 11, 2004·Biochemical and Biophysical Research Communications·Donghong JuYouming Xie
Oct 27, 2004·The Journal of Biological Chemistry·Li WangYouming Xie
Dec 4, 2004·Genome Biology·Astrid C HaugenBennett Van Houten
Feb 24, 2006·The Journal of Biological Chemistry·Donghong Ju, Youming Xie
Sep 23, 2008·FEBS Letters·Dmitry S KarpovVadim L Karpov

❮ Previous
Next ❯

Citations

Sep 30, 2016·Critical Reviews in Biochemistry and Molecular Biology·Ravikiran S YedidiCordula Enenkel
Mar 12, 2020·Applied Microbiology and Biotechnology·Julia A BubisDmitry S Karpov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.