The transcription elongation factor CA150 interacts with RNA polymerase II and the pre-mRNA splicing factor SF1

Molecular and Cellular Biology
Aaron C GoldstrohmMariano A Garcia-Blanco

Abstract

CA150 represses RNA polymerase II (RNAPII) transcription by inhibiting the elongation of transcripts. The FF repeat domains of CA150 bind directly to the phosphorylated carboxyl-terminal domain of the largest subunit of RNAPII. We determined that this interaction is required for efficient CA150-mediated repression of transcription from the alpha(4)-integrin promoter. Additional functional determinants, namely, the WW1 and WW2 domains of CA150, were also required for efficient repression. A protein that interacted directly with CA150 WW1 and WW2 was identified as the splicing-transcription factor SF1. Previous studies have demonstrated a role for SF1 in transcription repression, and we found that binding of the CA150 WW1 and WW2 domains to SF1 correlated exactly with the functional contribution of these domains for repression. The binding specificity of the CA150 WW domains was found to be unique in comparison to known classes of WW domains. Furthermore, the CA150 binding site, within the carboxyl-terminal half of SF1, contains a novel type of proline-rich motif that may be recognized by the CA150 WW1 and WW2 domains. These results support a model for the recruitment of CA150 to repress transcription elongation. In this model, C...Continue Reading

References

Apr 1, 1995·Current Opinion in Genetics & Development·D L Bentley
Jul 1, 1994·DNA and Cell Biology·C De MeirsmanJ J Cassiman
Apr 1, 1993·Trends in Biochemical Sciences·A L Greenleaf
Jan 19, 1996·The Journal of Biological Chemistry·J P Richardson
May 1, 1996·Molecular and Cellular Biology·J BlauD Bentley
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·A YuryevJ L Corden
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M J MortillaroR Berezney
Aug 9, 1996·The Journal of Biological Chemistry·M E Dahmus
Jan 1, 1996·Annual Review of Biochemistry·A Krämer
Nov 1, 1996·Genes & Development·G OrphanidesD Reinberg
Jan 1, 1996·Progress in Biophysics and Molecular Biology·M Sudol
Apr 1, 1997·Current Opinion in Genetics & Development·A ShilatifardR C Conaway
Jan 1, 1997·Annual Review of Biochemistry·S M UptainM J Chamberlin
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·P CramerA R Kornblihtt
Feb 7, 1998·Genes & Development·K M Neugebauer, M B Roth
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·M Hampsey
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·M T BedfordP Leder
Mar 6, 1999·The Journal of Biological Chemistry·Y H Ping, T M Rana
Mar 13, 1999·The Journal of Biological Chemistry·Y YamaguchiH Handa

❮ Previous
Next ❯

Citations

Jul 21, 2012·Cellular and Molecular Life Sciences : CMLS·Hansjörg RindtChristian L Lorson
Oct 17, 2002·Journal of Molecular Biology·Mark AllenMark Bycroft
Aug 18, 2009·Nature Structural & Molecular Biology·Schraga SchwartzGil Ast
Oct 20, 2010·The Journal of Biological Chemistry·Jie ZhouRichard G Pestell
Mar 29, 2012·The Journal of Biological Chemistry·Noemí Sánchez-HernándezCarlos Suñé
Jan 14, 2005·Molecular Biology of the Cell·Goranka Tanackovic, Angela Krämer
Feb 4, 2010·Nucleic Acids Research·Kuo-Ming LeeWoan-Yuh Tarn
Aug 15, 2002·Genome Research·Juri RappsilberMatthias Mann
Apr 21, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Ankit Gupta, Clara L Kielkopf
Oct 16, 2004·Molecular and Cellular Biology·Matthew J SmithTony Pawson
Aug 2, 2005·Molecular and Cellular Biology·Robert J InghamTony Pawson
Feb 28, 2013·Development·Kumari PushpaKuppuswamy Subramaniam
Sep 24, 2004·RNA·Alberto R KornblihttGuadalupe Nogues
Jul 5, 2013·PloS One·Shijia ZhuYadong Wang
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·John Karanicolas, Charles L Brooks
Mar 29, 2013·Genes and Immunity·C WangA-C Syvänen
Oct 24, 2015·Wiley Interdisciplinary Reviews. RNA·Soraya BecerraCarlos Suñé
Aug 13, 2015·Journal of Cellular Biochemistry·Nicholas J MillerWilliam J Roesler
Oct 30, 2015·RNA Biology·Mohamed Hafez, Georg Hausner
Apr 28, 2012·Gene·Marta MontesCarlos Suñé
Aug 21, 2013·Chemical Reviews·Dirk Eick, Matthias Geyer
Nov 18, 2011·Molecular Biology International·Michael C Yu
Mar 29, 2008·Current Opinion in Genetics & Development·Eric AllemandChristian Muchardt
Oct 1, 2015·Nucleic Acids Research·Angela CrisciAngela Krämer
Sep 29, 2007·Cancer Science·Miki ShitashigeTesshi Yamada
Jun 11, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Zerrin FidanRudolf Volkmer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.