The transcription factor FcWRKY40 of Fortunella crassifolia functions positively in salt tolerance through modulation of ion homeostasis and proline biosynthesis by directly regulating SOS2 and P5CS1 homologs

The New Phytologist
Wenshan DaiJi-Hong Liu

Abstract

Although some WRKYs have been characterized, regulatory roles of most WRKYs remain poorly understood. Herein, we elucidated function of FcWRKY40 from Fortunella crassifolia in salt tolerance via overexpression and virus-induced gene silencing (VIGS) and unraveled its target genes. Overexpression of FcWRKY40 enhanced salt tolerance in transgenic tobacco and lemon, while silencing of FcWRKY40 increased salt susceptibility. Homolog genes of Salt Overly Sensitive 2 (SOS2) and Δ-1-pyrroline-5-carboxylate synthetase 1 (P5CS1) were dramatically up-regulated in transgenic lemon but down-regulated in VIGS line. Consistently, transgenic lemon displayed lower Na+ and higher proline concentrations, whereas the silenced line accumulated more Na+ but less proline. Treatment of transgenic lemon with 24-epi-brassinolide compromised salt tolerance, while supply of exogenous proline partially restored salt tolerance of the VIGS line. FcWRKY40 specifically binds to and activates promoters of FcSOS2 and FcP5CS1. FcWRKY40 was up-regulated by ABA and salt, and confirmed as a target of ABA-responsive element binding factor 2 (FcABF2). Moreover, salt treatment up-regulated FcABF2 and FcP5CS1, and elevated proline concentrations. Taken together, our fi...Continue Reading

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Citations

Aug 25, 2019·International Journal of Molecular Sciences·Hongqiu ZengHaitao Shi
Feb 23, 2020·International Journal of Molecular Sciences·Xiaorui LiJinyin Lv
Dec 3, 2020·The Plant Journal : for Cell and Molecular Biology·Yuanzhong JiangHuanhuan Liu
Mar 6, 2021·Frontiers in Plant Science·Xiaomin LiuChao Li
Aug 11, 2020·Plant Science : an International Journal of Experimental Plant Biology·Zhenyu HeJuan Xu
Sep 10, 2020·Plant Science : an International Journal of Experimental Plant Biology·Qinglong DongFengwang Ma

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