The transcription factor NF-kappaB/p50 interacts with the blk gene during B cell activation.

The Journal of Biological Chemistry
Patty ZwolloM E Koshland

Abstract

The B cell-specific transcription factor Pax-5 has been shown previously to interact with the promoter of the blk gene in vitro. blk encodes a tyrosine kinase associated with the B cell receptor, which is expressed during the early but not the final stages of B cell development. To investigate whether Pax-5 regulates expression of the blk gene in vivo during B cell development and/or activation, Pax-5a was overexpressed in B cell lines. Increases in blk promoter activity using a chloramphenicol acetyltransferase reporter gene system suggested a role for Pax-5a as a transcriptional activator. Subsequent site-specific mutagenesis studies showed that mutations of the Pax-5 binding site on blk significantly alter promoter activity, although results suggested that other factors could bind to this region as well. Using mobility shift assays, we detected an inducible transcription factor that interacts strongly with a sequence overlapping the Pax-5 site on the blk promoter and identified this as a homodimer of NF-kappaB/p50, a member of the NF-kappaB/Rel family of transcription factors. This factor was present at high levels in lipopolysaccharide-activated normal B cells and in plasma cell lines but either at low levels or undetectabl...Continue Reading

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Citations

Dec 12, 2012·Archives of Environmental Contamination and Toxicology·Catherine A LewisPatty Zwollo
Jun 26, 2013·Developmental and Comparative Immunology·Elizabeth MacMurrayPatty Zwollo
Feb 25, 2000·Molecular and Cellular Biology·S Maitra, M Atchison
Apr 9, 2009·Blood·Thorbjørn KrejsgaardNiels Ødum
Aug 14, 2010·Developmental and Comparative Immunology·Patty ZwolloMaggie Barr
Mar 9, 2004·Biochemical and Biophysical Research Communications·Ruth C BorghaeiJoanna Woloshin
Jun 6, 2014·Journal of Virology·Aimee N ReedLing Jin
Dec 28, 2018·European Journal of Clinical Investigation·Chao-Wen ChengJiunn-Diann Lin
Sep 6, 2001·The Journal of Biological Chemistry·M LowenP Zwollo
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patty ZwolloStephen Kaattari
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J AnspachP Zwollo
Feb 17, 2010·Human Immunology·Orlando TorresMiguel A Gonzalez-Gay

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