The transcription factor SOX30 is a key regulator of mouse spermiogenesis

Development
Daoqin ZhangChunsheng Han

Abstract

The postmeiotic development of male germ cells, also known as spermiogenesis, features the coordinated expression of a large number of spermatid-specific genes. However, only a limited number of key transcription factors have been identified and the underlying regulatory mechanisms remain largely unknown. Here, we report that SOX30, the most-divergent member of the Sry-related high-motility group box (SOX) family of transcription factors, is essential for mouse spermiogenesis. The SOX30 protein was predominantly expressed in spermatids, while its transcription was regulated by retinoic acid and by MYBL1 before and during meiosis. Sox30 knockout mice arrested spermiogenesis at step 3 round spermatids, which underwent apoptosis and abnormal chromocenter formation. We also determined that SOX30 regulated the expression of hundreds of spermatid-specific protein-coding and long non-coding RNA genes. SOX30 bound to the proximal promoter of its own gene and activated its transcription. These results reveal SOX30 as a novel key regulator of spermiogenesis that regulates its own transcription to enforce and activate this meiotic regulatory pathway.

References

Jun 11, 1992·Nucleic Acids Research·P DennyA Ashworth
May 9, 1991·Nature·P KoopmanR Lovell-Badge
Feb 1, 1997·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A H PetersP de Boer
May 16, 2001·Science·D ZhangR G Roeder
Oct 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nikolaus SchultzDavid L Garbers
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chon-Hwa Tsai-MorrisMaria L Dufau
Aug 11, 2004·The Journal of Cell Biology·Mounia GuenatriGeneviève Almouzni
Aug 18, 2009·Methods in Molecular Biology·Emad A Ahmed, Dirk G de Rooij
Dec 17, 2009·BMC Developmental Biology·Gary C HorvathW Stephen Kistler
Mar 26, 2010·Human Molecular Genetics·Michael J W VanGompel, Eugene Yujun Xu
Dec 15, 2010·BMC Molecular Biology·Fei HanDeshou Wang
Apr 19, 2011·Trends in Genetics : TIG·Aline V Probst, Geneviève Almouzni
Jul 14, 2011·Development·Ewelina Bolcun-FilasJohn C Schimenti
Jan 10, 2013·PLoS Genetics·Rowena LaveryMarie-Christine Chaboissier
Jun 14, 2013·Nature Communications·Haiyun GanFuchou Tang
Jun 26, 2013·Cell Reports·Magali SoumillonHenrik Kaessmann
Oct 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Haiying ZhouRobert Tjian
Nov 26, 2013·The International Journal of Biochemistry & Cell Biology·Jiankui ZhouXingxu Huang
Jun 15, 2014·Methods in Molecular Biology·Makiko KomataMasashige Bando
Nov 25, 2014·Nature Cell Biology·Manuel ViottiAnna-Katerina Hadjantonakis
Apr 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·Tsutomu EndoDavid C Page
Jun 28, 2015·Genes & Development·Kaja A WasikGregory J Hannon
Jul 15, 2015·PLoS Genetics·W Stephen KistlerWalter Reith
Aug 8, 2015·Genesis : the Journal of Genetics and Development·William ShawlotRichard H Finnell

❮ Previous
Next ❯

Citations

Nov 7, 2019·International Journal of Molecular Sciences·Yaohao TangLing Wei
Oct 29, 2020·Asian Journal of Andrology·Rossella CannarellaAldo E Calogero

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis