The transcription factor Zeb2 regulates signaling in mast cells.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Emilia Alina BarbuReuben P Siraganian

Abstract

Mast cell activation results in the release of stored and newly synthesized inflammatory mediators. We found that Zeb2 (also named Sip1, Zfhx1b), a zinc finger transcription factor, regulates both early and late mast cell responses. Transfection with small interfering RNA (siRNA) reduced Zeb2 expression and resulted in decreased FcεRI-mediated degranulation, with a parallel reduction in receptor-induced activation of NFAT and NF-κB transcription factors, but an enhanced response to the LPS-mediated activation of NF-κB. There was variable and less of a decrease in the Ag-mediated release of the cytokines TNF-α, IL-13, and CCL-4. This suggests that low Zeb2 expression differentially regulates signaling pathways in mast cells. Multiple phosphorylation events were impaired that affected molecules both at early and late events in the signaling pathway. The Zeb2 siRNA-treated mast cells had altered cell cycle progression, as well as decreased expression of several molecules including cell surface FcεRI and its β subunit, Gab2, phospholipase-Cγ1, and phospholipase-Cγ2, all of which are required for receptor-induced signal transduction. The results indicate that the transcription factor Zeb2 controls the expression of molecules thereby...Continue Reading

References

Mar 21, 1998·The Journal of Biological Chemistry·Y S BaeS G Rhee
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·A A Postigo, D C Dean
Jun 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y KawakamiT Kawakami
Jul 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y KawakamiT Kawakami
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhi-Hui XieReuben P Siraganian
Sep 10, 2002·Molecular Immunology·Reuben P SiraganianKiyonao Sada
Jul 16, 2003·Trends in Immunology·Taro Fukao, Shigeo Koyasu
Nov 25, 2003·Current Opinion in Immunology·Reuben P Siraganian
Jul 2, 2004·The Journal of Biological Chemistry·Silvia MonticelliAnjana Rao
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yutaka KanamaruAtsuhito Nakao
Aug 2, 2005·European Journal of Medical Genetics·Christiane ZweierAnita Rauch
Nov 30, 2005·Nucleic Acids Research·Cindy VandewalleGeert Berx
Feb 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Min YuHaihua Gu
Apr 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuko FurumotoJuan Rivera
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthias KleinMichael Stassen
Nov 23, 2006·Immunobiology·Stefanie Klemm, Jürgen Ruland
Dec 22, 2006·Nature Reviews. Molecular Cell Biology·Neil D Perkins
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wai-Hang Leung, Silvia Bolland
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aurelia Walczak-DrzewieckaJaroslaw Dastych
Aug 1, 2008·Nature·Daehyun BaekDavid P Bartel
Aug 1, 2008·Nature·Matthias SelbachNikolaus Rajewsky
Sep 9, 2008·Trends in Immunology·Mi-Sun KimAlasdair M Gilfillan
Oct 22, 2008·Nature Immunology·Janet Kalesnikoff, Stephen J Galli
Nov 18, 2008·Cellular and Molecular Life Sciences : CMLS·C VandewalleG Berx
Dec 18, 2008·Nature Immunology·Monika Vig, Jean-Pierre Kinet
Apr 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mitsuyoshi UtsugiMasatomo Mori
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·A Emre SayanEugene Tulchinsky
Nov 17, 2009·Immunological Reviews·Taku KambayashiGary A Koretzky
Nov 17, 2009·Immunological Reviews·Damiana Alvarez-ErricoJuan Rivera
Feb 18, 2010·Cell Cycle·Gareth BrowneEugene Tulchinsky
Mar 26, 2010·The Journal of Biological Chemistry·Emilia Alina BarbuReuben P Siraganian
Mar 26, 2010·Nature Reviews. Immunology·William E Paul, Jinfang Zhu
May 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Juan ZhangReuben P Siraganian

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Citations

Jan 19, 2016·Frontiers in Immunology·Melissa Krystel-WhittemoreJohn G Wood
Jul 30, 2014·European Journal of Immunology·Riccardo SibilanoCarlo E Pucillo
Dec 12, 2017·F1000Research·Silvia Monticelli, Cristina Leoni
Aug 16, 2017·The Journal of Experimental Medicine·Gökhan CildirVinay Tergaonkar
May 15, 2019·Cells·Edoardo ParrellaMarina Pizzi
May 11, 2018·HemaSphere·Bieke SoenSteven Goossens
Jun 23, 2021·The Journal of Biological Chemistry·Jacob T JacksonSeth L Masters

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