The transcription factors Runx3 and ThPOK cross-regulate acquisition of cytotoxic function by human Th1 lymphocytes

ELife
Yasmina SerroukhArnaud Marchant

Abstract

Cytotoxic CD4 (CD4CTX) T cells are emerging as an important component of antiviral and antitumor immunity, but the molecular basis of their development remains poorly understood. In the context of human cytomegalovirus infection, a significant proportion of CD4 T cells displays cytotoxic functions. We observed that the transcriptional program of these cells was enriched in CD8 T cell lineage genes despite the absence of ThPOK downregulation. We further show that establishment of CD4CTX-specific transcriptional and epigenetic programs occurred in a stepwise fashion along the Th1-differentiation pathway. In vitro, prolonged activation of naive CD4 T cells in presence of Th1 polarizing cytokines led to the acquisition of perforin-dependent cytotoxic activity. This process was dependent on the Th1 transcription factor Runx3 and was limited by the sustained expression of ThPOK. This work elucidates the molecular program of human CD4CTX T cells and identifies potential targets for immunotherapy against viral infections and cancer.

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Citations

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Datasets Mentioned

BETA
GSE75406

Methods Mentioned

BETA
flow cytometry
PCR
gene knockdown
density gradient centrifugation
FACS
Assay
immunoprecipitation
nucleic acid extraction
transfection

Software Mentioned

MiXCR
GSEA
Perl scripts
DIVA
Gene set enrichment analysis ( GSEA )
tcR
pvclust
lumi
FlowJo
BubbleGUM

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