PMID: 11931351Apr 5, 2002Paper

The transcription of the hGnRH-I and hGnRH-II genes in human neuronal cells is differentially regulated by estrogen

Journal of Molecular Neuroscience : MN
Alon ChenYitzhak Koch

Abstract

Gonadotropin releasing hormone-I (GnRH-I), a decapeptide serves as a key regulator of reproduction. Recently, several groups have identified in the mammalian brain a second form of GnRH, of unknown function, designated GnRH-II. The human neuronal medulloblastoma cells (TE-671) were recently demonstrated to express the two forms of GnRH (GnRH-I and GnRH-II). We used this cell line, as a model system, to investigate the regulation of human GnRH-I and GnRH-II genes by estrogen. Estrogen is one of the principal regulators of GnRH-I in hypothalamic neurons, acting as a classic homeostatic feedback molecule between the gonads and the brain. In this study, we investigated the regulation of the two GnRH forms by estrogen, in the human neuronal cell line TE-671. We demonstrate, for the first time, that the hGnRH-II and hGnRH-I genes are differentially regulated by estrogen. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern hybridization, we found that estrogen increases endogenous hGnRH-II mRNA levels and decreases endogenous hGnRH-I mRNA levels. Furthermore, we found these effects to be promoter-mediated. We cloned the hGnRH-I and hGnRH-II promoter constructs upstream to a luciferase reporter plasmid, and cotr...Continue Reading

Citations

May 29, 2003·Molecular and Cellular Endocrinology·Peter C K LeungXiao-Ming Zhu
Jan 10, 2018·Frontiers in Endocrinology·Amy T DesaulniersBrett R White
Sep 24, 2005·Endocrinology·Deborah K BarnettDavid H Abbott
Aug 2, 2003·Biology of Reproduction·Sandra L PetersenClifford D Carpenter
Dec 21, 2005·Molecular Human Reproduction·Chung-Man YeungPeter C K Leung
Oct 31, 2008·The FEBS Journal·Vien H Y LeeBilly K C Chow

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