PMID: 9442384Jan 27, 1998Paper

The transcriptional activation pattern of lipopolysaccharide binding protein (LBP) involving transcription factors AP-1 and C/EBP beta

Immunobiology
Carsten J KirschningR R Schumann

Abstract

Lipopolysaccharide (LPS) Binding Protein (LBP) is an acute phase protein with the ability to recognize bacterial LPS and transport it to the CD14 molecule or into HDL particles. It is synthesized in hepatocytes and secreted into the blood stream. LBP levels significantly rise during the acute phase response and levels of LBP may be important for an appropriate host reaction to bacterial challenge and for developing the sepsis syndrome. In order to elucidate the mechanisms of LBP regulation we investigated its transcription pattern and performed promoter studies under experimental conditions mimicking an acute phase scenario. In human hepatoma cell lines stimulation with IL-1 beta, IL-6, TNF-alpha and dexamethasone leads to strong transcriptional activation of the LBP gene in a dose- and time-dependent manner. IL-6 alone induces LBP significantly, whereas IL-1 beta mainly increases the IL-6 effect when applied in combination. Our results furthermore show that AP-1 and C/EBP beta are transcription factors involved in the activation of the LBP gene, as revealed by Luciferase reporter gene analysis and electromobility shift assays. Elucidating the mechanism of transcriptional activation of LBP potentially may help in understanding ...Continue Reading

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Citations

Dec 22, 2015·Cellular & Molecular Immunology·Zhou ZhouBin Gao
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Jan 25, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·K L BidneA F Keating
Aug 1, 2002·The Journal of Biological Chemistry·Felizabel Garcia BannachLindsey A Miles
Apr 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kerstin WolkRobert Sabat

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