The transcriptional factors MurR and catabolite activator protein regulate N-acetylmuramic acid catabolism in Escherichia coli.

Journal of Bacteriology
Tina Jaeger, Christoph Mayer

Abstract

The MurNAc etherase MurQ of Escherichia coli is essential for the catabolism of the bacterial cell wall sugar N-acetylmuramic acid (MurNAc) obtained either from the environment or from the endogenous cell wall (i.e., recycling). High-level expression of murQ is required for growth on MurNAc as the sole source of carbon and energy, whereas constitutive low-level expression of murQ is sufficient for the recycling of peptidoglycan fragments continuously released from the cell wall during growth of the bacteria. Here we characterize for the first time the expression of murQ and its regulation by MurR, a member of the poorly characterized RpiR/AlsR family of transcriptional regulators. Deleting murR abolished the extensive lag phase observed for E. coli grown on MurNAc and enhanced murQ transcription some 20-fold. MurR forms a stable multimer (most likely a tetramer) and binds to two adjacent inverted repeats within an operator region. In this way MurR represses transcription from the murQ promoter and also interferes with its own transcription. MurNAc-6-phosphate, the substrate of MurQ, was identified as a specific inducer that weakens binding of MurR to the operator. Moreover, murQ transcription depends on the activation by cyclic...Continue Reading

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Citations

Jul 29, 2011·Applied Microbiology and Biotechnology·Jan Reith, Christoph Mayer
Mar 14, 2012·Biochemistry·Marc A BoudreauShahriar Mobashery
Sep 10, 2010·The Journal of Biological Chemistry·Silke LitzingerChristoph Mayer
Feb 12, 2013·Journal of Bacteriology·Michael E OlsonAlexander R Horswill
Jul 26, 2011·Journal of Bacteriology·Petra R A KohlerSilvia Rossbach
Nov 10, 2009·Bioscience, Biotechnology, and Biochemistry·Daiki IshiiKaneyoshi Yamamoto
Jul 24, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Feng LiShimin Zhao
Aug 13, 2009·The Biochemical Journal·Maria Veiga-da-CunhaEmile Van Schaftingen
Sep 23, 2014·Applied Microbiology and Biotechnology·Tamara Aleksandrzak-PiekarczykJacek Bardowski
May 16, 2017·Carbohydrate Research·Sandra UnsleberChristoph Mayer
Apr 8, 2014·Journal of Bacteriology·Nagender LedalaGreg A Somerville
Jul 13, 2019·International Journal of Medical Microbiology : IJMM·Christoph MayerMarina Borisova
Jul 18, 2015·Microbiology Spectrum·Anthony R RichardsonAbraham L Sonenshein

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