The transcriptional programme controlled by Runx1 during early embryonic blood development.

Developmental Biology
Yosuke TanakaBerthold Göttgens

Abstract

Transcription factors have long been recognised as powerful regulators of mammalian development yet it is largely unknown how individual key regulators operate within wider regulatory networks. Here we have used a combination of global gene expression and chromatin-immunoprecipitation approaches during the early stages of haematopoietic development to define the transcriptional programme controlled by Runx1, an essential regulator of blood cell specification. Integrated analysis of these complementary genome-wide datasets allowed us to construct a global regulatory network model, which suggested that key regulators are activated sequentially during blood specification, but will ultimately collaborate to control many haematopoietically expressed genes. Using the CD41/integrin alpha 2b gene as a model, cellular and in vivo studies showed that CD41 is controlled by both Scl/Tal1 and Runx1 in fully specified blood cells, and initiation of CD41 expression in E7.5 embryos is severely compromised in the absence of Runx1. Taken together, this study represents the first global analysis of the transcriptional programme controlled by any key haematopoietic regulator during the process of early blood cell specification. Moreover, the conce...Continue Reading

References

Feb 1, 1993·Genes & Development·D StottB G Herrmann
Feb 16, 2002·Journal of Hematotherapy & Stem Cell Research·Kyunghee Choi
Nov 25, 2003·Bioinformatics·Gabriel S EichlerDonald E Ingber
Dec 13, 2005·PLoS Biology·Saulius Sumanas, Shuo Lin
Feb 14, 2006·Science·Eric H Davidson, Douglas H Erwin
Feb 24, 2006·Genesis : the Journal of Genetics and Development·Igor M SamokhvalovAlexander Medvinsky
May 10, 2007·Nature Protocols·Vítezslav BryjaErnest Arenas
May 16, 2007·Development, Growth & Differentiation·Kazuaki HashimotoMinetaro Ogawa
Jun 2, 2007·Science·David S JohnsonBarbara Wold
Oct 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·John E PimandaBerthold Göttgens
Jan 11, 2008·Blood·Josette-Renée LandryBerthold Göttgens
Jun 3, 2008·Nucleic Acids Research·Fátima Al-ShahrourJoaquín Dopazo
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Dec 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eric H Davidson, Michael S Levine
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Oct 30, 2009·British Journal of Haematology·Katrin OttersbachBerthold Göttgens
Dec 19, 2009·PLoS Genetics·Peter C HollenhorstBarbara J Graves
Aug 17, 2010·The International Journal of Developmental Biology·Gemma SwiersNancy A Speck
Dec 21, 2010·Nature Cell Biology·Michelina IacovinoMichael Kyba
Dec 24, 2010·Science·UNKNOWN modENCODE ConsortiumManolis Kellis
Feb 25, 2011·Stem Cell Research & Therapy·Nicola K WilsonBerthold Göttgens

❮ Previous
Next ❯

Citations

Jan 28, 2014·Developmental and Comparative Immunology·Feng YueLinsheng Song
Aug 21, 2013·Blood Cells, Molecules & Diseases·Victoria MoignardBerthold Göttgens
Apr 27, 2013·Circulation Research·Kathrina L MarceloKaren K Hirschi
Aug 8, 2013·Briefings in Functional Genomics·Ellen V Rothenberg
Feb 6, 2016·Cellular and Molecular Life Sciences : CMLS·Emily Gritz, Karen K Hirschi
Mar 1, 2016·Developmental Cell·Debbie K GoodeConstanze Bonifer
May 13, 2014·Experimental Hematology·Koen H M PrangeJoost H A Martens
Mar 13, 2015·Blood·Berthold Göttgens
Oct 9, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Miguel G ToscanoPedro J Real
Jul 8, 2016·Nature·Antonio ScialdoneBerthold Göttgens
Nov 17, 2016·Stem Cells and Development·Daisuke SugiyamaAlistair R R Forrest
Sep 20, 2017·Journal of Cellular Physiology·Jessica L PhillipsAdele F Holloway
Feb 26, 2015·The EMBO Journal·Wataru Ebina, Derrick J Rossi
Jun 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Arif KirmizitasRoger Patient
Aug 23, 2019·FEBS Letters·Sara MenegattiValerie Kouskoff
Aug 10, 2019·Nature Communications·Florian BonkhoferRoger Patient

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE29112

Methods Mentioned

BETA
immunoprecipitation
ChIP
ChIP-Seq
FACS
flow cytometry
PCR
ChIPs
transgenic

Software Mentioned

FACS Aria
MACS
bowtie
Gene Expression Dynamics Inspector ( GEDI )
TFBSSearch
MEME
FlowJo

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.

Cell Fate Specification

Cell fate specification is determined by complex signal transduction pathways including Notch and the Sonic hedgehog signaling pathway. Here is the latest research on regulation of cell fate determination.