The transcriptome of early chicken embryos reveals signaling pathways governing rapid asymmetric cellularization and lineage segregation

Development
Young Sun HwangJae Yong Han

Abstract

The phylogenomics and comparative functional genomics of avian species were investigated in the Bird 10,000 Genomes (B10K) project because of the important evolutionary position of birds and their value as a research model. However, the systematic profiling of transcriptional changes prior to oviposition has not been investigated in avian species because of the practical difficulties in obtaining pre-oviposited eggs. In this study, a total of 137 pre-oviposited embryos were collected from hen ovaries and oviducts and subjected to RNA-sequencing analyses. Two waves of chicken zygotic genome activation (ZGA) were observed. Functionally distinct developmental programs involving Notch, MAPK, Wnt and TGFβ signaling were separately detected during cleavage and area pellucida formation. Furthermore, the early stages of chicken development were compared with the human and mouse counterparts, highlighting chicken-specific signaling pathways and gradually analogous gene expression via ZGA. These findings provide a genome-wide understanding of avian embryogenesis and comparisons among amniotes.

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Citations

Sep 11, 2019·Molecular Biology and Evolution·Yuuri YasuokaYasushi Okazaki
Nov 16, 2019·Cellular and Molecular Life Sciences : CMLS·Deivendran RengarajJae Yong Han
Jun 4, 2020·International Journal of Molecular Sciences·Joonbum LeeKichoon Lee
Mar 19, 2021·Genome Research·Peng LiuJianguo Xia
Jun 9, 2021·Progress in Biophysics and Molecular Biology·John S Torday

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