The transcriptome of Spodoptera exigua larvae exposed to different types of microbes

Insect Biochemistry and Molecular Biology
Laura PascualSalvador Herrero

Abstract

We have obtained and characterized the transcriptome of Spodoptera exigua larvae with special emphasis on pathogen-induced genes. In order to obtain a highly representative transcriptome, we have pooled RNA from diverse insect colonies, conditions and tissues. Sequenced cDNA included samples from 3 geographically different colonies. Enrichment of RNA from pathogen-related genes was accomplished by exposing larvae to different pathogenic and non-pathogenic microbial agents such as the bacteria Bacillus thuringiensis, Micrococcus luteus, and Escherichia coli, the yeast Saccharomyces cerevisiae, and the S. exigua nucleopolyhedrovirus (SeMNPV). In addition, to avoid the loss of tissue-specific genes we included cDNA from the midgut, fat body, hemocytes and integument derived from pathogen exposed insects. RNA obtained from the different types of samples was pooled, normalized and sequenced. Analysis of the sequences obtained using the Roche 454 FLX and Sanger methods has allowed the generation of the largest public set of ESTs from S. exigua, including a large group of immune genes, and the identification of an important number of SSR (simple sequence repeats) and SNVs (single nucleotide variants: SNPs and INDELs) with potential us...Continue Reading

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Citations

May 30, 2013·PLoS Pathogens·Agata K JakubowskaSalvador Herrero
Jul 18, 2014·PLoS Pathogens·Joël T van MierloRonald P van Rij
Jul 6, 2014·Journal of Invertebrate Pathology·Agata K JakubowskaSalvador Herrero
Jun 21, 2014·Developmental and Comparative Immunology·Anchalee TassanakajonPiti Amparyup
Jan 10, 2016·Toxicon : Official Journal of the International Society on Toxinology·Ji Hyeong BaekMin Gab Kim
Sep 4, 2014·Environmental Entomology·Youngjin ParkYonggyun Kim
Oct 6, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G Navarro-CerrilloS Herrero
Sep 4, 2015·Insect Molecular Biology·C Ayra-PardoDenis J Wright
Aug 17, 2014·Journal of Invertebrate Pathology·Cristina VirtoPrimitivo Caballero
Dec 23, 2016·Archives of Insect Biochemistry and Physiology·Shao-Liang FangChao-Liang Liu
Feb 2, 2016·PLoS Genetics·Clément GilbertElisabeth A Herniou
Sep 11, 2017·Applied Microbiology and Biotechnology·María Martínez-SolísSalvador Herrero
Aug 21, 2013·The Journal of Experimental Biology·Youngjin Park, Yonggyun Kim

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