The transcriptome-wide landscape and modalities of EJC binding in adult Drosophila

BioRxiv : the Preprint Server for Biology
Ales ObrdlikAnne Ephrussi

Abstract

Splicing-dependent assembly of the exon junction complex (EJC) at canonical sites -20 to -24 nucleotides upstream of exon-exon junctions in mRNAs occurs in all higher eukaryotes and affects most major regulatory events in the life of a transcript. In mammalian cell cytoplasm, EJC is essential for efficient RNA surveillance, while in Drosophila the most essential cytoplasmic EJC function is in localization of oskar mRNA. Here we developed a method for isolation of protein complexes and associated RNA-targets (ipaRt), which provides a transcriptome-wide view of RNA binding sites of the fully assembled EJC in adult Drosophila . We find that EJC binds at canonical positions, with highest occupancy on mRNAs from genes comprising multiple splice sites and long introns. Moreover, the occupancy is highest at junctions adjacent to strong splice sites, CG-rich hexamers and RNA structures. These modalities have not been identified by previous studies in mammals, where more binding was seen at non-canonical positions. The most highly occupied transcripts in Drosophila have increased tendency to be maternally localized, and are more likely to derive from genes involved in differentiation or development. Taken together, we identify the RNA m...Continue Reading

Related Concepts

Carrageenan
Cell Differentiation Process
Cytoplasm
Drosophila
Exons
Genes
Nucleotides
RNA
RNA, Messenger
Adjacent

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