The transcriptomics of glucocorticoid receptor signaling in developing zebrafish

PloS One
Dinushan Nesan, Mathilakath M Vijayan

Abstract

Cortisol is the primary corticosteroid in teleosts that is released in response to stressor activation of the hypothalamus-pituitary-interrenal axis. The target tissue action of this hormone is primarily mediated by the intracellular glucocorticoid receptor (GR), a ligand-bound transcription factor. In developing zebrafish (Danio rerio) embryos, GR transcripts and cortisol are maternally deposited into the oocyte prior to fertilization and influence early embryogenesis. To better understand of the molecular mechanisms involved, we investigated changes in the developmental transcriptome prior to hatch, in response to morpholino oligonucleotide knockdown of GR using the Agilent zebrafish microarray platform. A total of 1313 and 836 mRNA transcripts were significantly changed at 24 and 36 hours post fertilization (hpf), respectively. Functional analysis revealed numerous developmental processes under GR regulation, including neurogenesis, eye development, skeletal and cardiac muscle formation. Together, this study underscores a critical role for glucocorticoid signaling in programming molecular events essential for zebrafish development.

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Citations

Mar 5, 2016·Scientific Reports·Dinushan Nesan, Mathilakath M Vijayan
Sep 24, 2015·Aquatic Toxicology·Oana BirceanuMathilakath M Vijayan
Sep 24, 2015·Molecular Human Reproduction·Liangliang SunNorman J Dovichi
Jan 18, 2017·Scientific Reports·Carol BestMathilakath M Vijayan
Jan 10, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Andrew AntolicMaureen Keller-Wood
Dec 10, 2019·General and Comparative Endocrinology·Colette VaillantElisabeth Pellegrini

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Datasets Mentioned

BETA
GSE50376

Methods Mentioned

BETA
Feature Extraction
chip
PCR

Software Mentioned

Ingenuity Pathway Analysis
Agilent Feature Extraction
Agilent GeneSpring
R
Ingenuity Pathway Analysis ( IPA )
Ingenuity
IPA
R Statistical Computing environment

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