The Drosophila transient receptor potential protein (TRP) and its mammalian homologues are thought to be Ca(2+)-permeable cation channels activated by G protein (G(q/11))-coupled receptors and are regarded as an interesting molecular model for the Ca(2+) entry mechanisms associated with stimulated phosphoinositide turnover and store depletion. However, there is little unequivocal evidence linking mammalian TRPs with particular native functions. In this study, we have found that heterologous expression of murine TRP6 in HEK293 cells reproduces almost exactly the essential biophysical and pharmacological properties of alpha(1)-adrenoceptor-activated nonselective cation channels (alpha(1)-AR-NSCC) previously identified in rabbit portal vein smooth muscle. Such properties include activation by diacylglycerol; S-shaped current-voltage relationship; high divalent cation permeability; unitary conductance of 25 to 30 pS and augmentation by flufenamate and Ca(2+); and blockade by Cd(2+), La(3+), Gd(3+), SK&F96365, and amiloride. Reverse transcriptase-polymerase chain reaction and confocal laser scanning microscopy using TRP6-specific primers and antisera revealed that the level of TRP6 mRNA expression was remarkably high in both murine ...Continue Reading
Ion-concentration dependence of the reversal potential and the single channel conductance of ion channels at the frog neuromuscular junction
Ins(1,4,5)P3 activates Drosophila cation channel Trpl in recombinant baculovirus-infected Sf9 insect cells
Pharmacological characterization of muscarinic receptor-activated cation channels in guinea-pig ileum
Dual regulation of cation-selective channels by muscarinic and alpha 1-adrenergic receptors in the rabbit portal vein
Novel Ca2+ channels underlying transduction in Drosophila photoreceptors: implications for phosphoinositide-mediated Ca2+ mobilization
Dual effect of external Ca2+ on noradrenaline-activated cation current in rabbit portal vein smooth muscle cells
Fenamates potentiate the alpha 1-adrenoceptor-activated nonselective cation channels in rabbit portal vein smooth muscle
On the molecular basis and regulation of cellular capacitative calcium entry: roles for Trp proteins
Alpha 1-adrenoceptor activation of a non-selective cation current in rabbit portal vein by 1,2-diacyl-sn-glycerol
Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion
Cloning and expression of a novel mammalian homolog of Drosophila transient receptor potential (Trp) involved in calcium entry secondary to activation of receptors coupled by the Gq class of G protein.
Molecular cloning and functional characterization of a novel receptor-activated TRP Ca2+ channel from mouse brain.
Physiological features of visceral smooth muscle cells, with special reference to receptors and ion channels
Facilitatory effect of Ca2+ on the noradrenaline-evoked cation current in rabbit portal vein smooth muscle cells
Activation of heterologously expressed Drosophila TRPL channels: Ca2+ is not required and InsP3 is not sufficient
Molecular and functional characterization of a novel mouse transient receptor potential protein homologue TRP7. Ca(2+)-permeable cation channel that is constitutively activated and enhanced by stimulation of G protein-coupled receptor.
Evidence for a role of Trp proteins in the oxidative stress-induced membrane conductances of porcine aortic endothelial cells
Regulation of TRPM2 channels in neutrophil granulocytes by ADP-ribose: a promising pharmacological target
Functional characterization and physiological relevance of the TRPC3/6/7 subfamily of cation channels
Vascular activity of two silicon compounds, ALIS 409 and ALIS 421, novel multidrug-resistance reverting agents in cancer cells
Extracellular Mg(2+) blocks endothelin-1-induced contraction through the inhibition of non-selective cation channels in coronary smooth muscle
Multiple roles of calmodulin and other Ca(2+)-binding proteins in the functional regulation of TRP channels
The diacylgylcerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance
Effect of chronic hypoxia on voltage-independent calcium influx activated by 5-HT in rat intrapulmonary arteries
Ketamine blocks voltage-gated K(+) channels and causes membrane depolarization in rat mesenteric artery myocytes
Nonselective cation channels are essential for maintaining intracellular Ca2+ levels and spontaneous firing activity in the midbrain dopamine neurons
PKD1 haploinsufficiency is associated with altered vascular reactivity and abnormal calcium signaling in the mouse aorta.
Examining the role of mechanosensitive ion channels in pressure mechanotransduction in rat bladder urothelial cells
Expression levels of TRPC1 and TRPC6 ion channels are reciprocally altered in aging rat aorta: implications for age-related vasospastic disorders.
Activation of TRPC4β by Gαi subunit increases Ca2+ selectivity and controls neurite morphogenesis in cultured hippocampal neuron
Mechanisms for L-channel-mediated increase in [Ca(2+)]i and its reduction by anti-bipolar drugs in cultured astrocytes combined with its mRNA expression in freshly isolated cells support the importance of astrocytic L-channels
Involvement of IP3-receptor activation in endothelin-1-induced Ca(2+) influx in rat pulmonary small artery
M3-like muscarinic receptors mediate Ca2+ influx in rat mesencephalic GABAergic neurones through a protein kinase C-dependent mechanism
N-terminal truncation of human alpha1D-adrenoceptors increases expression of binding sites but not protein
Fulfenamic acid sensitive, Ca(2+)-dependent inward current induced by nicotinic acetylcholine receptors in dopamine neurons
The TRP family of cation channels: probing and advancing the concepts on receptor-activated calcium entry
The developing relationship between receptor-operated and store-operated calcium channels in smooth muscle
20-Hydroxyeicosatetraenoic acid potentiates stretch-induced contraction of canine basilar artery via PKC alpha-mediated inhibition of KCa channel
Lysophosphatidylcholine, a component of atherogenic lipoproteins, induces the change of calcium mobilization via TRPC ion channels in cultured human corporal smooth muscle cells
Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound.
Reactive oxygen species-mediated TRPC6 protein activation in vascular myocytes, a mechanism for vasoconstrictor-regulated vascular tone.
Identification of ML204, a novel potent antagonist that selectively modulates native TRPC4/C5 ion channels.
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