The transmembrane domain of subunit b of the Escherichia coli F1F(O) ATP synthase is sufficient for H(+)-translocating activity together with subunits a and c

European Journal of Biochemistry
Jörg-Christian GreieK Altendorf

Abstract

Subunit b is indispensable for the formation of a functional H(+)-translocating F(O) complex both in vivo and in vitro. Whereas the very C-terminus of subunit b interacts with F(1) and plays a crucial role in enzyme assembly, the C-terminal region is also considered to be necessary for proper reconstitution of F(O) into liposomes. Here, we show that a synthetic peptide, residues 1-34 of subunit b (b(1-34)) [Dmitriev, O., Jones, P.C., Jiang, W. & Fillingame, R.H. (1999) J. Biol. Chem.274, 15598-15604], corresponding to the membrane domain of subunit b was sufficient in forming an active F(O) complex when coreconstituted with purified ac subcomplex. H(+) translocation was shown to be sensitive to the specific inhibitor N,N'-dicyclohexylcarbodiimide, and the resulting F(O) complexes were deficient in binding of isolated F(1). This demonstrates that only the membrane part of subunit b is sufficient, as well as necessary, for H(+) translocation across the membrane, whereas the binding of F(1) to F(O) is mainly triggered by C-terminal residues beyond Glu34 in subunit b. Comparison of the data with former reconstitution experiments additionally indicated that parts of the hydrophilic portion of the subunit b dimer are not involved in ...Continue Reading

References

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Citations

Jan 22, 2008·Journal of Bioenergetics and Biomembranes·Amanda K WelchBrian D Cain
Nov 28, 2007·Cell Research·Xiaojin LiHuizhou Fan
Sep 29, 2009·Nature Structural & Molecular Biology·Denys PogoryelovThomas Meier
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lindsay A BakerJohn L Rubinstein
Jun 6, 2009·Annual Review of Biochemistry·Christoph von BallmoosPeter Dimroth
Jun 25, 2008·Annual Review of Biophysics·Christoph von BallmoosPeter Dimroth
Aug 18, 2009·Chemistry and Physics of Lipids·Natalie Bordag, Sandro Keller
Oct 19, 2005·Advances in Protein Chemistry·Stephan Wilkens
Oct 5, 2014·Journal of Molecular and Cellular Cardiology·Muhammad Rizwan AlamMichel Ovize
Apr 4, 2008·The FEBS Journal·Thomas VorburgerChristoph von Ballmoos
Jan 13, 2017·Journal of Bioenergetics and Biomembranes·Robert R IshmukhametovSteven B Vik
Apr 20, 2018·Frontiers in Physiology·Alain DautantRoza Kucharczyk
Apr 1, 2007·EcoSal Plus·Steven B Vik

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