PMID: 9557679Apr 29, 1998Paper

The transmembrane domains of Sindbis virus envelope glycoproteins induce cell death

Journal of Virology
A K JoeB Levine

Abstract

Sindbis virus, the prototype alphavirus, kills cells by inducing apoptosis. To investigate potential mechanisms by which Sindbis virus induces apoptosis, we examined whether specific viral gene products were able to induce cell death. Genes encoding the three structural proteins--capsid, the precursor E1 (6K plus E1), and the precursor E2 (P62 or E3 plus E2)--were cotransfected with a beta-galactosidase reporter plasmid in transient-transfection assays in rat prostate adenocarcinoma AT3 cells. Cell death, as determined by measuring the loss of blue cells, was observed in AT3 cells transfected with 6K plus E1 and with P62 but not in cells transfected with capsid. Deletion mutagenesis of P62 indicated that large regions of the cytoplasmic domain and extracellular domain were not essential for the induction of cell death. However, constructs containing the minimal E3 signal sequence fused to the E2 transmembrane domain and the minimal E3 signal sequence fused to the E1 transmembrane domain induced death as efficiently as full-length P62 and 6K plus E1, whereas no cell death was observed after transfection with a control construct containing the E3 signal sequence linked to the transmembrane domain of murine CD4. These data demonst...Continue Reading

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Citations

May 7, 2002·Journal of Virology·Grigori G Prikhod'koJeffrey I Cohen
Aug 16, 2013·DNA and Cell Biology·Kristina M Giorda, Daniel N Hebert
Jul 4, 2001·FEBS Letters·M C ParquetA Igarashi
Nov 5, 1999·Annual Review of Microbiology·A RoulstonP E Branton
Jun 21, 2002·The Journal of General Virology·Stewart Hay, George Kannourakis
Dec 24, 2009·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Erin ReichertJoseph Larsen
Jun 10, 2015·Virus Genes·Wulin ShaozhouYun Zhang
Dec 24, 2003·International Reviews of Immunology·Mei-Ling Li, Victor Stollar
Sep 3, 2009·Future Microbiology·Joyce JoseRichard J Kuhn
Apr 28, 1999·Trends in Microbiology·D E Griffin, J M Hardwick
Oct 12, 2000·Clinical Microbiology Reviews·J Y Lee, D S Bowden

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis