PMID: 1201276Dec 16, 1975Paper

The transmembrane proteins in the plasma membrane of normal human erythrocytes. Evaluation employing lactoperoxidase and proteases

Biochemistry
T J Mueller, M Morrison

Abstract

The molecular architecture of the human erythrocyte membrane has been probed using lactoperoxidase-catalyzed iodination in conjunction with Pronase hydrolysis. Resealed, hemoglobin-free ghosts were labeled at the cytoplasmic surface and the external membrane surface was subsequently digested with Pronase. Changes in size of the components labeled at the cytoplasmic surface were readily detected by sodium dodecyl sulfate gel electrophoresis. The protein 3 molecular weight class labeled at the cytoplasmic surface was extensively hydrolyzed at the external surface to produce a major 65000 molecular weight fragment and a minor 45000 molecular weight fragment. When resealed membranes were labeled on the external surface the same 65000 molecular weight labeled component is produced. These results unequivocally demonstrate that the same polypeptides in the protein 3 molecular weight class that can be labeled by lactoperoxidase at the cytoplasmic membrane surface are digested by Pronase at the external surface and are, therefore, transmembrane components. Where it is possible to label one surface of a membrane with lactoperoxidase and reseal the membrane this procedure represents an alternate method for establishing transmembrane confi...Continue Reading

Citations

Oct 1, 1977·The Journal of Membrane Biology·K F Schnell
Mar 28, 1980·Biochemical and Biophysical Research Communications·J BrandtL O Andersson
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H FukushimaH Hasekura
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A Lalazar, A Loyter
Mar 8, 1979·Biochimica Et Biophysica Acta·Y LasterA Loyter
Nov 1, 1983·Archives of Biochemistry and Biophysics·L Hsu, M Morrison
Mar 1, 1979·Archives of Biochemistry and Biophysics·D Harell, M Morrison
Jul 1, 1980·Biochimica Et Biophysica Acta·N K ChemerisV I Geletyuk
Feb 10, 1987·Biochemistry·J A ThompsonD D Cunningham
Jan 1, 1980·CRC Critical Reviews in Biochemistry·P J Quinn, D Chapman

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