The transmembrane segment of Tom20 is recognized by Mim1 for docking to the mitochondrial TOM complex

Journal of Molecular Biology
Joanne M HulettTrevor Lithgow

Abstract

Mitochondria cannot be made de novo. Mitochondrial biogenesis requires that up to 1000 proteins are imported into mitochondria, and the protein import pathway relies on hetero-oligomeric translocase complexes in both the inner and outer mitochondrial membranes. The translocase in the outer membrane, the TOM complex, is composed of a core complex formed from the beta-barrel channel Tom40 and additional subunits each with single, alpha-helical transmembrane segments. How alpha-helical transmembrane segments might be assembled onto a transmembrane beta-barrel in the context of a membrane environment is a question of fundamental importance. The master receptor subunit of the TOM complex, Tom20, recognizes the targeting sequence on incoming mitochondrial precursor proteins, binds these protein ligands, and then transfers them to the core complex for translocation across the outer membrane. Here we show that the transmembrane segment of Tom20 contains critical residues essential for docking the Tom20 receptor into its correct environment within the TOM complex. This crucial docking reaction is catalyzed by the unique assembly factor Mim1/Tom13. Mutations in the transmembrane segment that destabilize Tom20, or deletion of Mim1, preven...Continue Reading

References

Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·T LithgowG Schatz
Jul 12, 1993·FEBS Letters·M MoczkoN Pfanner
Jun 23, 2001·Protein Science : a Publication of the Protein Society·N S GreenK N Houk
Jul 26, 2002·Nature·Guri GiaeverMark Johnston
Aug 15, 2003·The Journal of Biological Chemistry·Thomas WaizeneggerDoron Rapaport
Oct 23, 2003·The Journal of Biological Chemistry·Vera KozjakNikolaus Pfanner
Dec 20, 2003·Nature·Stefan A PaschenWalter Neupert
Dec 31, 2003·The Journal of Cell Biology·Ian GentleTrevor Lithgow
May 18, 2004·Research in Microbiology·Romé Voulhoux, Jan Tommassen
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Jul 10, 2004·Cell·Sanie MnaimnehTimothy R Hughes
Aug 25, 2004·The Journal of Cell Biology·Daigo IshikawaToshiya Endo
Nov 4, 2004·Nature Structural & Molecular Biology·Nikolaus PfannerTrevor Lithgow
Dec 21, 2004·EMBO Reports·Thomas WaizeneggerDoron Rapaport
Jan 15, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Billy T DyePaul Ahlquist
Aug 12, 2005·Molecular Membrane Biology·Ryan ListerJames Whelan
Nov 30, 2005·Molecular Microbiology·Ian E GentleTrevor Lithgow
Feb 8, 2006·Current Biology : CB·Andrew J PerryPaul R Gooley
Jun 9, 2006·The Journal of Biological Chemistry·Chris MeisingerNikolaus Pfanner
Jul 22, 2006·Science·Pavel DolezalTrevor Lithgow
Dec 2, 2006·Nature Reviews. Molecular Cell Biology·Gunnar von Heijne
Feb 1, 2007·Annual Review of Biochemistry·Walter Neupert, Johannes M Herrmann

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Citations

Aug 24, 2010·Nature Reviews. Molecular Cell Biology·Oliver SchmidtChris Meisinger
Aug 25, 2012·Molecular Biology of the Cell·Maria BohnertMartin van der Laan
Nov 18, 2010·Molecular Biology and Evolution·Janette TongTrevor Lithgow
Sep 6, 2013·Neurochemical Research·Emmanouela KallergiKostas Tokatlidis
Apr 30, 2013·The Journal of Biological Chemistry·Thomas BeckerNikolaus Pfanner
Aug 10, 2011·The Journal of Cell Biology·Thomas BeckerNikolaus Pfanner
Aug 10, 2011·The Journal of Cell Biology·Drazen PapicDoron Rapaport
Feb 14, 2015·Journal of Molecular Biology·Walter Neupert
Oct 12, 2014·Biochimica Et Biophysica Acta·Alexandra I C HöhrNils Wiedemann
Dec 1, 2009·European Journal of Cell Biology·Kai Stefan Dimmer, Doron Rapaport
May 9, 2009·Current Opinion in Cell Biology·Thomas BeckerMartin van der Laan
Aug 22, 2015·Current Opinion in Structural Biology·Maria BohnertMartin van der Laan
Jan 24, 2013·IUBMB Life·Aksana VarabyovaAgnieszka Chacinska
Dec 18, 2014·Protein Science : a Publication of the Protein Society·Susanne E HorvathNikolaus Pfanner
Sep 6, 2011·Biochimica Et Biophysica Acta·Kai Stefan Dimmer, Doron Rapaport
Feb 25, 2014·Cell Metabolism·Angelika B HarbauerChris Meisinger
Jun 12, 2012·Biochimica Et Biophysica Acta·Jan DudekMartin van der Laan
Jul 6, 2010·Biochimica Et Biophysica Acta·Jovana Dukanovic, Doron Rapaport
Nov 23, 2010·Journal of Molecular Biology·Kieran A RimmerPaul R Gooley
Apr 22, 2008·Biochimica Et Biophysica Acta·Thomas BeckerChris Meisinger
Jan 31, 2015·Biochimica Et Biophysica Acta·Lena-Sophie WenzThomas Becker
Dec 20, 2011·Trends in Biochemical Sciences·Thomas BeckerNikolaus Pfanner
May 27, 2008·Biochimica Et Biophysica Acta·Dirk M Walther, Doron Rapaport
Sep 7, 2013·Progress in Lipid Research·Susanne E Horvath, Günther Daum
Aug 26, 2009·Cell·Agnieszka ChacinskaNikolaus Pfanner
Aug 11, 2010·Biochimica Et Biophysica Acta·Natalia GebertDiana Stojanovski
Dec 1, 2009·Biochimica Et Biophysica Acta·Toshiya Endo, Koji Yamano
Feb 19, 2015·Journal of Bioenergetics and Biomembranes·Lena BöttingerThomas Becker
Nov 5, 2016·Biochimica Et Biophysica Acta. Molecular Cell Research·Michal WasilewskiAgnieszka Chacinska
Mar 17, 2017·Annual Review of Biochemistry·Nils Wiedemann, Nikolaus Pfanner
Sep 17, 2017·The Journal of Cell Biology·Vivien KrügerChris Meisinger
Dec 2, 2017·PloS One·Subbulakshmi SureshStephen A Osmani

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