The transmembrane transporter domain of glutamate transporters is a process tip localizer

Scientific Reports
Mariko Kato Hayashi, Masato Yasui

Abstract

Glutamate transporters in the central nervous system remove glutamate released from neurons to terminate the signal. These transporters localize to astrocyte process tips approaching neuronal synapses. The mechanisms underlying the localization of glutamate transporters to these processes, however, are not known. In this study, we demonstrate that the trimeric transmembrane transporter domain fragment of glutamate transporters, lacking both N- and C-terminal cytoplasmic regions, localized to filopodia tips. This is a common property of trimeric transporters including a neutral amino acid transporter ASCT1. Astrocyte specific proteins are not required for the filopodia tip localization. An extracellular loop at the centre of the 4(th) transmembrane helices, unique for metazoans, is required for the localization. Moreover, a C186S mutation at the 4(th) transmembrane region of EAAT1, found in episodic ataxia patients, significantly decreased its process tip localization. The transmembrane transporter domain fragments of glutamate transporters also localized to astrocyte process tips in cultured hippocampal slice. These results indicate that the transmembrane transporter domain of glutamate transporters have an additional function ...Continue Reading

References

Sep 1, 1994·Neuron·J D RothsteinR W Kuncl
Feb 28, 2002·Molecular and Cellular Neurosciences·Hélène MarieDavid Attwell
Jun 25, 2004·Nucleic Acids Research·Burkhard RostJinfeng Liu
Jul 16, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jianzheng Zhou, Margaret L Sutherland
Dec 9, 2004·Journal of Neuroscience Methods·Adrienne M BenediktssonMichael E Dailey
Sep 1, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michael HaberKeith K Murai
Jun 15, 2007·Journal of Pharmacological Sciences·Ryuta KoyamaYuji Ikegaya
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Nov 8, 2007·Nature Reviews. Neuroscience·Anastassios V Tzingounis, Jacques I Wadiche
Jan 11, 2008·The European Journal of Neuroscience·Merav BassanPaul A Rosenberg
Feb 6, 2008·Journal of Neurochemistry·Inmaculada M González-GonzálezFrancisco Zafra
Apr 23, 2008·Molecular Membrane Biology·Matjaz StenovecRobert Zorec
Jan 14, 2009·Archives of Neurology·Boukje de VriesArn M J M van den Maagdenberg
Jun 26, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Erin M RoseDavid R Hampson
Feb 24, 2010·Brain Research Reviews·Andreas ReichenbachFrank Kirchhoff
Mar 17, 2010·Pflügers Archiv : European journal of physiology·Anthony Lau, Michael Tymianski
Sep 16, 2010·Nature Reviews. Neuroscience·Giles E Hardingham, Hilmar Bading
Nov 26, 2010·The Journal of Biological Chemistry·Ariane LeinenweberChristoph Fahlke
Mar 24, 2011·Journal of Neurochemistry·Shengwei ZouArnold Eskin
Sep 9, 2011·IUBMB Life·Esther López-Bayghen, Arturo Ortega
Nov 5, 2011·Glia·Adrienne M BenediktssonMichael E Dailey
Dec 16, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Elizabeth N GendaMichael B Robinson
Feb 4, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ming-Chi TsaiJacques I Wadiche
Feb 7, 2012·Neurochemistry International·Deborah E BauerMichael B Robinson
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 6, 2012·European Journal of Pharmacology·Ankita MehtaP L Sharma

❮ Previous
Next ❯

Citations

Jun 20, 2016·Molecular Neurobiology·Mariangela ChisariMaria Angela Sortino
Mar 4, 2017·International Journal of Molecular Sciences·Laura M Frago, Julie A Chowen
Apr 13, 2018·International Journal of Molecular Sciences·Mariko Kato Hayashi
Oct 27, 2017·Scientific Reports·Peter KovermannTobias Freilinger
Jun 13, 2019·Journal of Neurochemistry·Mariko Kato HayashiKaoru Sato

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
PCR
transfection

Software Mentioned

ImageJ
Fluoview

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.