The transplanted fetal endocrine pancreas undergoes an inherent sequential differentiation similar to that in the native pancreas. An ultrastructural study in the pig-to-mouse model

Diabetes
A Lukinius, O Korsgren

Abstract

This study examines, at the ultrastructural level, whether the fetal porcine endocrine pancreas (insulin, glucagon, somatostatin, and pancreatic polypeptide [PP]- and islet amyloid polypeptide [IAPP]-containing cells) develops normally after transplantation under the kidney capsule in athymic mice. We have thus used an in vivo pig-to-mouse model for the differentiation of the endocrine pancreas removed from its normal milieu. Islet-like cell clusters (ICCs) were prepared from the fetal porcine pancreas as previously described and transplanted under the renal capsule of athymic mice. At various times after transplantation, the endocrine pancreas was removed and the level of differentiation was compared with the native pancreas of the same biological age. At the ultrastructural level, several sequential steps could be identified based on the morphology and hormone content of the secretory granules of the endocrine cell examined. Applying this approach, we could demonstrate that the ontogeny of the transplanted fetal pig pancreas follows the same sequential differentiation as the native pancreas. The process seems to be under stringent control, apparently directly related to the biological age of the tissue, and independent not on...Continue Reading

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Citations

Dec 18, 2001·Diabetes/metabolism Research and Reviews·B NazlielB Koçer
Oct 10, 2002·Xenotransplantation·Philip O'Connell
Sep 26, 2003·Transplantation·Debra A MacKenzieHans W Sollinger
Mar 11, 2003·Scandinavian Cardiovascular Journal : SCJ·Christina Nyström-RosanderStefan Thelin
May 2, 2008·Xenotransplantation·Gregory S Korbutt
Dec 7, 2006·The Biochemical Journal·Tiago BragaGunnar Pejler
Sep 12, 2021·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth KemterMichele Solimena

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