The transport mechanism of integrin αvβ3 receptor targeting nanoparticles in Caco-2 cells

International Journal of Pharmaceutics
Yining XuYuan Huang

Abstract

As for the existence of epithelium barrier, accelerating the transport remains huge challenges for orally delivered protein and peptide drugs into blood circulation. Modifying nanopaticles (NPs) with targeting peptides can enhance the intestinal absorption of loaded macromolecular drugs. However, the transport process, which mainly means how the NPs pass through the apical membrane and the basolateral side and then enter into blood circulation, is needed comprehensive investigation. In this study, we systemically studied the transport mechanisms in Caco-2 cell model of trimethyl chitosan based NPs (TMC NPs) before and after modification of FQS, an integrin αvβ3 receptor targeting peptide. Our results showed FQS peptide mediated multiple endocytosis pathways and could activate integrin αvβ3 receptor by interacting with FAK and Src-family kinases to induce receptor-mediated endocytosis of the NPs. Then, both endocytosed NPs could transport from early endosome to lysososmes via late endosomes/lysosome pathway, as well as to recycling endosomes and Golgi apparatus through early endosome/recycling endosomes and Golgi apparatus/recycling endosomes/plasma membrane pathways, respectively. After FQS peptide modification, the endocytosis...Continue Reading

References

Feb 1, 1995·Current Opinion in Cell Biology·N B Cole, J Lippincott-Schwartz
Dec 1, 1993·The Journal of Cell Biology·F AnientoJ Gruenberg
Jun 17, 1998·The Journal of Biological Chemistry·A P FeranchakJ G Fitz
Feb 26, 2000·Molecular Biology of the Cell·C BucciB van Deurs
Jul 4, 2001·The Journal of Biological Chemistry·J Y LiouK K Wu
Aug 11, 2001·Biochemical Society Transactions·J P LuzioR C Piper
Sep 29, 2001·Advanced Drug Delivery Reviews·M ThanouH E Junginger
Oct 25, 2003·Journal of Cell Science·Jan SchmoranzerSanford M Simon
Nov 6, 2003·Journal of Cell Science·Ben Nichols
Jun 5, 2004·Journal of Theoretical Biology·Abraham R TzafririElazer R Edelman
Nov 27, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ikuhiko NakaseShiroh Futaki
May 27, 2006·Cancer Letters·Michael Z Gilcrease
Aug 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bianka L GrosshansPeter Novick
Oct 20, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anne des RieuxVéronique Préat
Jul 31, 2007·Advanced Drug Delivery Reviews·Lisa M Bareford, Peter W Swaan
Nov 17, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Florence Delie, María José Blanco-Príeto
May 27, 2008·Biomaterials·Omathanu P PerumalRangaramanujam M Kannan
Feb 24, 2009·Biomaterials·Malgorzata S CartieraW Mark Saltzman
May 19, 2009·Nanomedicine : Nanotechnology, Biology, and Medicine·Mohammad Reza AvadiMorteza Rafiee-Tehrani
Mar 17, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gaurav SahayAlexander V Kabanov
Oct 16, 2010·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Miguel Quintela-FandinoRamon Colomer
Mar 5, 2013·Advanced Drug Delivery Reviews·Anne des RieuxVéronique Préat

❮ Previous
Next ❯

Citations

Apr 28, 2016·Advanced Drug Delivery Reviews·Ana BeloquiVéronique Préat
Jun 14, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Abhijit A DateLaura M Ensign
Mar 5, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuli BaiYuan Huang
Nov 23, 2017·Frontiers in Pharmacology·Xin LiuXuehui Cai
Sep 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Min LiuYuan Huang
Jan 27, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Liyun XingYuan Huang
Mar 7, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shunsuke YamaguchiSumio Ohtsuki
Feb 23, 2021·Asian Journal of Pharmaceutical Sciences·Huan WangChangyou Zhan
Feb 28, 2018·ACS Applied Materials & Interfaces·Yining XuYuan Huang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.