PMID: 9420219Jan 7, 1998Paper

The tripartite leader sequence of subgroup C adenovirus major late mRNAs can increase the efficiency of mRNA export

Journal of Virology
W Huang, S J Flint

Abstract

The subgroup C human adenoviruses induce selective export of newly synthesized viral mRNA from the nucleus to the cytoplasm, with concomitant inhibition of export of the majority of cellular mRNA species. Such posttranscriptional regulation of viral and cellular gene expression in infected cells requires viral E1B and E4 proteins. To facilitate the investigation of parameters that govern selective export in adenovirus-infected cells, we constructed a marked human beta-actin minigene under the control of the glucocorticoid-inducible enhancer-promoter of mouse mammary tumor virus and introduced it into the left end of the adenovirus type 5 (Ad5) genome. Transcription of this reporter gene (designated MA) as well as of a sibling, which differed only in the inclusion of a cDNA copy of the Ad2 major late tripartite leader sequence upstream of beta-actin sequences (termed MtplA), in recombinant virus-infected cells was strictly dependent on the addition of dexamethasone to the medium. When transcription of the MA gene was induced during the late phase of infection, newly synthesized MA RNA entered the cytoplasm. These transcripts, which contain no viral sequences, therefore reproduce the behavior of exceptional cellular mRNA species ...Continue Reading

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Citations

Feb 24, 2001·The Journal of Gene Medicine·L GraveM Mehtali
Jun 29, 2004·World Journal of Gastroenterology : WJG·Li LiWen-Lin Huang
Nov 3, 2006·Journal of Virology·Jennifer L Woo, Arnold J Berk
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Jan 29, 2009·Protein Expression and Purification·Irina L TutykhinaAlexander L Gintsburg
May 24, 2011·Journal of Virological Methods·Xiao-Hui ZouTao Hung

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