PMID: 7542780Jul 18, 1995Paper

The tRNA processing enzyme RNase T is essential for maturation of 5S RNA

Proceedings of the National Academy of Sciences of the United States of America
Z Li, M P Deutscher

Abstract

The maturation of 5S RNA in Escherichia coli is poorly understood. Although it is known that large precursors of 5S RNA accumulate in mutant cells lacking the endoribonuclease-RNase E, almost nothing is known about how the mature 5' and 3' termini of these molecules are generated. We have examined 5S RNA maturation in wild-type and single- or multiple-exoribonuclease-deficient cells by Northern blot and primer-extension analysis. Our results indicate that no mature 5S RNA is made in RNase T-deficient strains. Rather, 5S RNA precursors containing predominantly 2 extra nucleotides at the 3' end accumulate. Apparently, these 5S RNAs are functional inasmuch as mutant cells are viable, growing only slightly slower than wild type. Purified RNase T can remove the extra 3' residues, showing that it is directly involved in the trimming reaction. In contrast, mutations affecting other 3' exoribonucleases have no effect on 5S RNA maturation. Approximately 90% of the 5S RNAs in both wild-type and RNase T- cells contain mature 5' termini, indicating that 5' processing is independent of RNase T action. These data identify the enzyme responsible for generating the mature 3' terminus of 5S RNA molecules and also demonstrate that a completely p...Continue Reading

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Citations

Dec 10, 2002·Biochemical and Biophysical Research Communications·Franziska Klein, Elena Evguenieva-Hackenberg
Sep 2, 2003·Journal of Biotechnology·Juan F MartínMónica Barriuso
Aug 16, 2012·The Journal of Biological Chemistry·Tanmay DuttaMurray P Deutscher
Feb 28, 2002·The EMBO Journal·Zhongwei LiMurray P Deutscher
Dec 20, 2002·Nucleic Acids Research·Ciarán Condon, Harald Putzer
Feb 3, 2006·Nucleic Acids Research·Murray P Deutscher
Jan 31, 2012·Nucleic Acids Research·Bijoy K MohantySidney R Kushner
Dec 30, 2009·Journal of Bacteriology·Nancy S Gutgsell, Chaitanya Jain
Oct 16, 2012·Journal of Bacteriology·Nancy S Gutgsell, Chaitanya Jain
Sep 2, 2003·Journal of Bacteriology·Josh S Sharp, David H Bechhofer
Nov 5, 2008·Molecular and Cellular Biology·Yu-Yuan HsiaoHanna S Yuan
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Magdalena Kaczanowska, Monica Rydén-Aulin
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Ciarán Condon
May 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Zhuan-Fen Cheng, Murray P Deutscher
May 3, 2011·Annual Review of Biochemistry·Zahra ShajaniJames R Williamson
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z LiM P Deutscher
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z LiM P Deutscher
Feb 15, 2011·Nature Chemical Biology·Yu-Yuan HsiaoHanna S Yuan
Sep 13, 2015·Protein Science : a Publication of the Protein Society·Yulander DuhHanna S Yuan
Jul 25, 2009·Molecular Microbiology·Fernando Garza-SánchezChristopher S Hayes
Jan 19, 2007·Molecular Microbiology·Robert A BrittonCiarán Condon
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Jul 28, 2007·FEMS Microbiology Reviews·Christophe Pichon, Brice Felden
Jul 28, 2010·FEMS Microbiology Reviews·Cecília M ArraianoSandra C Viegas
Feb 26, 2000·Annual Review of Genetics·J Venema, D Tollervey
Apr 17, 2015·FEMS Microbiology Reviews·Murray P Deutscher

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