The TRPM1 channel in ON-bipolar cells is gated by both the α and the βγ subunits of the G-protein Go

Scientific Reports
Ying XuNoga Vardi

Abstract

Transmission from photoreceptors to ON bipolar cells in mammalian retina is mediated by a sign-inverting cascade. Upon binding glutamate, the metabotropic glutamate receptor mGluR6 activates the heterotrimeric G-protein Gαoβ3γ13, and this leads to closure of the TRPM1 channel (melastatin). TRPM1 is thought to be constitutively open, but the mechanism that leads to its closure is unclear. We investigated this question in mouse rod bipolar cells by dialyzing reagents that modify the activity of either Gαo or Gβγ and then observing their effects on the basal holding current. After opening the TRPM1 channels with light, a constitutively active mutant of Gαo closed the channel, but wild-type Gαo did not. After closing the channels by dark adaptation, phosducin or inactive Gαo (both sequester Gβγ) opened the channel while the active mutant of Gαo did not. Co-immunoprecipitation showed that TRPM1 interacts with Gβ3 and with the active and inactive forms of Gαo. Furthermore, bioluminescent energy transfer assays indicated that while Gαo interacts with both the N- and the C- termini of TRPM1, Gβγ interacts only with the N-terminus. Our physiological and biochemical results suggest that both Gαo and Gβγ bind TRPM1 channels and cooperate ...Continue Reading

References

Dec 1, 1992·Molecular Biology of the Cell·M Bomsel, K Mostov
Nov 22, 1990·Proceedings. Biological Sciences·R A Shiells, G Falk
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyA G Gilman
May 1, 1993·Visual Neuroscience·N VardiP Sterling
May 17, 1996·The Journal of Biological Chemistry·S MüllerM J Lohse
Apr 17, 1997·Biochemical and Biophysical Research Communications·F Chen, R H Lee
Jul 1, 1997·Visual Neuroscience·N Vardi, K Morigiwa
Jun 28, 2000·The Journal of Comparative Neurology·N VardiP Sterling
Oct 12, 2001·Trends in Endocrinology and Metabolism : TEM·N Dascal
Jan 13, 2006·British Journal of Pharmacology·Graeme Milligan, Evi Kostenis
Jun 21, 2007·Annual Review of Biochemistry·Kartik Venkatachalam, Craig Montell
Sep 18, 2007·The Journal of Biological Chemistry·Radda RusinovaDiomedes E Logothetis
Nov 29, 2007·Nature Reviews. Molecular Cell Biology·William M Oldham, Heidi E Hamm
Sep 5, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ying XuNoga Vardi
Oct 7, 2008·Annual Review of Pharmacology and Toxicology·Denis J DupréTerence E Hébert
Feb 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Moshe ParnasBaruch Minke
May 15, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yin ShenScott Nawy
Jul 25, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yan CaoKirill A Martemyanov
Oct 29, 2009·Proceedings of the National Academy of Sciences of the United States of America·Catherine W MorgansR Lane Brown
Nov 10, 2009·American Journal of Human Genetics·Maria M van GenderenMaarten Kamermans
Nov 10, 2009·American Journal of Human Genetics·Isabelle AudoChristina Zeitz
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chieko KoikeTakahisa Furukawa
Sep 15, 2010·The Journal of General Physiology·Haruhisa OkawaAlapakkam P Sampath
Sep 18, 2010·Cell Calcium·Chieko KoikeTakahisa Furukawa
Feb 4, 2011·Advances in Experimental Medicine and Biology·Elena Oancea, Nadine L Wicks
Mar 18, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Anuradha DhingraNoga Vardi
Jul 8, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jillian N PearringRonald G Gregg
Aug 13, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yan CaoKirill A Martemyanov
Dec 2, 2011·Journal of Neurophysiology·Ying XuNoga Vardi
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yin ShenScott Nawy
Aug 17, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Anuradha DhingraNoga Vardi
Aug 13, 2014·The Journal of Biological Chemistry·Melina A AgostoTheodore G Wensel

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Citations

Jan 4, 2018·International Journal of Molecular Sciences·Chang-Hoon ChoJae-Yong Park
Aug 23, 2017·ELife·Talisia QualloStuart Bevan
Feb 3, 2019·Journal of Cellular Physiology·Kah Keng WongSiti Muhamad Nur Husna
Mar 20, 2020·Investigative Ophthalmology & Visual Science·Moritz LindnerMark W Hankins
Oct 6, 2019·Cellular and Molecular Life Sciences : CMLS·Takahisa FurukawaYoshihiro Omori
Jul 19, 2017·Annual Review of Vision Science·Kirill A Martemyanov, Alapakkam P Sampath
Oct 31, 2020·Proceedings of the National Academy of Sciences of the United States of America·Marc BehrendtJohannes Oberwinkler

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Methods Mentioned

BETA
co-immunoprecipitation
myristoylation
pull-down
BRET
transfection
nucleotide exchange
co-IP

Software Mentioned

PatchMaster
Clampfit

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