The 'true' L-xylulose reductase of filamentous fungi identified in Aspergillus niger

FEBS Letters
Dominik MojzitaPeter Richard

Abstract

L-Xylulose reductase is part of the eukaryotic pathway for l-arabinose catabolism. A previously identified L-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on L-arabinose and the deletion strain showed no reduced L-xylulose reductase activity but instead lost the D-mannitol dehydrogenase activity. In this communication we identified the 'TRUE'L-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on L-arabinose and the deletion results in a strain lacking the NADPH-specific L-xylulose reductase activity and having reduced growth on l-arabinose. The purified enzyme had a K(m) for L-xylulose of 25 mM and a nu(max) of 650 U/mg.

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Citations

Jan 8, 2011·Applied Microbiology and Biotechnology·Bernhard Seiboth, Benjamin Metz
Apr 13, 2011·Applied Microbiology and Biotechnology·Evy BattagliaRonald P de Vries
Dec 17, 2015·Applied Microbiology and Biotechnology·Sun-Mi LeeHal S Alper
Oct 16, 2012·FEBS Letters·Birgit S GrubenRonald P de Vries
Apr 15, 2018·Current Genetics·Kana IshikawaTetsuo Kobayashi
Sep 1, 2019·International Journal of Biological Macromolecules·A MuruganP Chellapandi
Mar 6, 2021·Microbial Biotechnology·Tania ChroumpiRonald P de Vries

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