The Trypanosoma brucei AIR9-like protein is cytoskeleton-associated and is required for nucleus positioning and accurate cleavage furrow placement.

Molecular Microbiology
Sophie F MayTansy C Hammarton

Abstract

AIR9 is a cytoskeleton-associated protein in Arabidopsis thaliana with roles in cytokinesis and cross wall maturation, and reported homologues in land plants and excavate protists, including trypanosomatids. We show that the Trypanosoma brucei AIR9-like protein, TbAIR9, is also cytoskeleton-associated and colocalizes with the subpellicular microtubules. We find it to be expressed in all life cycle stages and show that it is essential for normal proliferation of trypanosomes in vitro. Depletion of TbAIR9 from procyclic trypanosomes resulted in increased cell length due to increased microtubule extension at the cell posterior. Additionally, the nucleus was re-positioned to a location posterior to the kinetoplast, leading to defects in cytokinesis and the generation of aberrant progeny. In contrast, in bloodstream trypanosomes, depletion of TbAIR9 had little effect on nucleus positioning, but resulted in aberrant cleavage furrow placement and the generation of non-equivalent daughter cells following cytokinesis. Our data provide insight into the control of nucleus positioning in this important pathogen and emphasize differences in the cytoskeleton and cell cycle control between two life cycle stages of the T. brucei parasite.

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Citations

May 3, 2013·Open Biology·Bungo Akiyoshi, Keith Gull
Dec 5, 2013·Molecular and Biochemical Parasitology·Cécile GalletLinda Kohl
Apr 5, 2013·The Plant Journal : for Cell and Molecular Biology·John Gardiner
Mar 3, 2017·PLoS Pathogens·Michael P RoutMark C Field
May 22, 2018·Molecular Microbiology·Nicholas A HiltonChristopher L de Graffenried
Dec 12, 2019·Frontiers in Cellular and Infection Microbiology·Tansy C Hammarton
Sep 1, 2019·Trends in Parasitology·Amy N Sinclair, Christopher L de Graffenried

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Datasets Mentioned

BETA
FR750404

Methods Mentioned

BETA
fluorescence microscopy
transmission electron microscopy
flow cytometry
electron microscopy
transfection
flow

Software Mentioned

SoftWoRx
Olympus SIS
OpenLab
RNAit
Improvision
Velocity

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