Abstract
Trypanosoma rangeli has recently been divided in two primary lineages denoted as KP1(+) and KP1(-) strains because of epidemiological and evolutionary interest in the molecular differentiation of these two groups. We report the molecular characterization of the genes encoding histone H2A protein from a T. rangeli KP1(+) strain (H14), its comparison to T. rangeli KP1(-) strain (C23) histone H2A coding genes [Puerta, C., Cuervo, P., Thomas, M.C., López, M.C., 2000. Molecular characterization of the histone H2A gene from the parasite Trypanosoma rangeli. Parasitol. Res. 86, 916-922], and its application in a low-stringency single specific primer polymerase chain reaction (LSSP-PCR) assay to differentiate these parasite groups. The results show that the locus encoding the H2A protein in the H14 strain is formed by at least 11 gene units measuring 799 nucleotides in length, organized in tandem, and located in two chromosomes of approximately 1.9 and 1.1Mb in size. Remarkably, in KP1(-) strains these genes are on pairs of chromosomes of about 1.7 and 1.9Mb. In addition, there is a hybridization signal in the compression region above 2.1Mb in all T. rangeli strains. Therefore, the chromosomal location of these genes is a useful marker...Continue Reading
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