PMID: 8969464Jun 1, 1996Paper

The Tryptophan oxygenase gene of Anopheles gambiae

Insect Biochemistry and Molecular Biology
O MukabayireN J Besansky

Abstract

The Anopheles gambiae gene encoding tryptophan oxygenase, a homolog of the Drosophila melanogaster vermilion gene, has been molecularly cloned and characterized. Unlike Drosophila, where it is X-linked, the A. gambiae gene maps to chromosome 2R, subdivision 12E, by in situ hybridization to the polytene chromosomes. Of the six introns present, four are positioned identically to those of the Drosophila homolog, one is similarly positioned, and one is novel. A 1 955 nt cDNA potentially encodes a 392 amino acid protein of an estimated 45 kDa. Amino acid comparisons between the deduced protein and previously known tryptophan oxygenases revealed 74% identity between Anopheles and Drosophila, and 53% identity between Anopheles and nematode or mammalian proteins. Northern analysis detected a developmentally regulated transcript about 2 kb in length. Since this gene is known to control adult eye color in other flies, its cloning from A. gambiae provides the basis for a dominant phenotypic marker for germline transformation, one whose expression, unlike that of white, is not cell autonomous.

References

Jul 16, 1990·Biochemical and Biophysical Research Communications·K MaezonoT Nakamura
Nov 1, 1995·Insect Molecular Biology·N J BesanskyF H Collins
Jan 1, 1996·Medical and Veterinary Entomology·M Q Benedict, H Chang

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Citations

Nov 21, 2007·Journal of Molecular Evolution·Hajime Julie YuasaTomohiko Suzuki
Apr 30, 2005·Journal of Insect Science·Jeffrey A FabrickJames E Baker
Feb 8, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hajime Julie Yuasa, Tomohiko Suzuki
Oct 31, 2006·Journal of Insect Physiology·Qian HanJianyong Li
Jul 14, 1998·Insect Molecular Biology·M AshburnerJ J Peloquin
Mar 31, 2017·Medical Oncology·Cheng-Peng YuDa-Ya Luo
Apr 14, 2016·Metabolic Brain Disease·Cheng-Peng YuDa-Ya Luo

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