The TTF-1/TAP26 complex differentially modulates surfactant protein-B (SP-B) and -C (SP-C) promoters in lung cells

Biochemical and Biophysical Research Communications
Meng-Chun YangYih-Sheng Yang

Abstract

Surfactant protein-B (SP-B) and -C (SP-C) are small hydrophobic surfactant proteins that maintain surface tension in alveoli. Both SP-B and SP-C are regulated by a key factor, thyroid transcription factor-1 (TTF-1), in lung cells. Previously, we identified a 26-kDa, TTF-1-associated protein (TAP26) that was shown to interact with TTF-1 and enhance TTF-1-transactivated SP-B promoter activity. In this study, we hypothesized that TAP26 could also serve as a co-activator of the SP-C promoter. Using the chromatin immunoprecipitation assay (ChIP), we demonstrated that TAP26 was not only a component of the SP-B promoter, but was also a component of the SP-C promoter complex in lung cells. TAP26 could synergistically stimulate TTF-1-activated SP-B and SP-C promoter activities in H441 cells (a lung adenocarcinoma cell). However, in MLE12 cells (a murine lung type II cell), only SP-B, but not SP-C, promoter activity was improved by TAP26 in a concentration-dependent manner. This result indicated that the TTF-1/TAP26 complex-activated SP-C promoter activity was already optimized in MLE12 cells and that the response of the SP-C promoter to the complex was different from that of the SP-B promoter. Via promoter mutation analysis, adjacent TT...Continue Reading

References

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Citations

Mar 3, 2010·American Journal of Respiratory and Critical Care Medicine·Yusuke TakahashiEiji Ikeda
Jul 29, 2008·Biochemical and Biophysical Research Communications·Yuhong GuoYih-Sheng Yang
Jul 10, 2007·Biochemical and Biophysical Research Communications·Yuhong GuoYih-Sheng Yang
Nov 26, 2015·PLoS Computational Biology·Minzhe GuoYan Xu
Feb 13, 2015·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Fei-Tao DengXin-Qun Chai
Sep 14, 2017·International Journal of Molecular Medicine·Yuan-Dong ChenHong-Yan Lu

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