The Tubulation Activity of a Fission Yeast F-BAR Protein Is Dispensable for Its Function in Cytokinesis

Cell Reports
Nathan A McDonaldKathleen L Gould

Abstract

F-BAR proteins link cellular membranes to the actin cytoskeleton in many biological processes. Here we investigated the function of the Schizosaccharomyces pombe Imp2 F-BAR domain in cytokinesis and find that it is critical for Imp2's role in contractile ring constriction and disassembly. To understand mechanistically how the F-BAR domain functions, we determined its structure, elucidated how it interacts with membranes, and identified an interaction between dimers that allows helical oligomerization and membrane tubulation. Using mutations that block either membrane binding or tubulation, we find that membrane binding is required for Imp2's cytokinetic function but that oligomerization and tubulation, activities often deemed central to F-BAR protein function, are dispensable. Accordingly, F-BARs that do not have the capacity to tubulate membranes functionally substitute for the Imp2 F-BAR, establishing that its major role is as a cell-cycle-regulated bridge between the membrane and Imp2 protein partners, rather than as a driver of membrane curvature.

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Citations

Jun 2, 2016·Cell Cycle·Nathan A McDonald, Kathleen L Gould
May 8, 2016·Cellular Microbiology·Pilar PérezJuan C Ribas
Dec 6, 2016·Acta Crystallographica. Section D, Structural Biology·Michael SpornyYarden Opatowsky
Sep 16, 2017·ELife·Nathan A McDonaldKathleen L Gould
Dec 7, 2018·The Journal of Cell Biology·Wilton T SneadJeanne C Stachowiak
Jun 19, 2019·Journal of Cell Science·MariaSanta C Mangione, Kathleen L Gould
Aug 5, 2018·The Journal of Cell Biology·Mikael V GarabedianBruce L Goode
Oct 28, 2019·Pharmacological Reports : PR·Begoña Quintana-VillamandosEmilio Delgado-Baeza
Sep 26, 2019·Molecular Biology of the Cell·Katherine L Schutt, James B Moseley
Feb 1, 2020·Pharmacological Reports : PR·Begoña Quintana-VillamandosEmilio Delgado-Baeza
Apr 24, 2021·Trends in Cell Biology·Chloe E SniderShiro Suetsugu
Aug 21, 2021·Cancer Gene Therapy·Sungjin ParkMyung-Haing Cho

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Methods Mentioned

BETA
X-ray
electron microscopy
PCR

Software Mentioned

Dali
HYSS
COR Odyssey
HKL2000
softWoRx
RESOLVE
Coot
PHENIX
REFMAC5
LI

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