The tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate enhances the proliferative response of Balb/c-3T3 cells to hormonal growth factors

Journal of Cellular Physiology
C N FrantzC D Scher

Abstract

Stimulation of Balb/c-3T3 cell growth by TPA requires factors found in serum. We examined the interaction between TPA and serum growth factors in the stimulation of cell growth. The number of cells synthesizing DNA (incorporating 3H-thymidine) within 24 to 30 hours after the addition of TPA and the growth factors to density-inhibited Balb/c-3T3 cultures in serum-free medium was determined by autoradiography. With no additions or with TPA (30--300 ng/ml) alone, only 3--7% of cells synthesized DNA. However, TPA synergistically promoted DNA synthesis in combination with each of the defined serum growth fractions, platelet derived growth factor and platelet poor plasma. TPA also synergistically promoted DNA synthesis in combination with purified growth factors including fibroblast growth factor, insulin (10(-6)--10(-5)M), and epidermal growth factor. In all conditions, TPA enhancement of DNA synthesis also resulted in an increase in cell number. Because TPA synergistically enhanced the activity of each growth factor tested, it did not act identically to any of the growth factors.

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W J PledgerC D Scher
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W J PledgerC D Scher
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H N AntoniadesC D Stiles
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C D StilesW J Pledger
Jun 1, 1978·The Journal of Clinical Endocrinology and Metabolism·G E DaileyD N Orth
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H N Antoniades, C D Scher
Jun 1, 1978·Journal of Cellular Physiology·J MoroneyC E Wenner
Jun 1, 1976·Journal of Cellular Physiology·G Carpenter, S Cohen
Jan 22, 1976·Nature·M Wigler, I B Weinstein
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R RossL Harker
Aug 1, 1974·Experimental Cell Research·N Kohler, A Lipton
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J A Smith, L Martin
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D Gospodarowicz, J S Moran
Jan 1, 1974·CRC Critical Reviews in Toxicology·R K Boutwell
Dec 1, 1965·Journal of Cellular Physiology·G J TodaroH Green

❮ Previous
Next ❯

Citations

Apr 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·M KurobeK Hayashi
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S D Balk
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D R ClemmonsW J Pledger
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N H ColburnG Abruzzo
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T G O'Brien, K Krzeminski
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J B Smith
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B FriedmanM R Rosner
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R J Davis, M P Czech
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N E OlashawW J Pledger
Oct 1, 1992·Molecular Biology of the Cell·N E OlashawW J Pledger
Apr 1, 1981·The Journal of Clinical Investigation·C K OsborneJ Ziegler
Mar 25, 2015·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Kiyoshi SasakiNoriho Tanaka
Jun 1, 1985·Experimental Cell Research·C N Frantz
Apr 1, 1981·Journal of Cellular Physiology·E Butler-Gralla, H R Herschman
Sep 1, 1981·Journal of Cellular Physiology·L M MatrisianB E Magun
Dec 1, 1989·Journal of Cellular Physiology·C J Morgan, W J Pledger
Dec 1, 1990·Journal of Cellular Physiology·M J SmythW Wharton
Feb 1, 1986·Journal of Cellular Physiology·B L EideD F Bowen-Pope
Mar 1, 1986·Journal of Cellular Physiology·C D ScherW Wharton
Jan 1, 1980·Journal of Supramolecular Structure·C D StilesC D Scher
Feb 11, 1981·Molecular and Cellular Biochemistry·E D Adamson, A R Rees
Aug 1, 1983·Molecular and Cellular Endocrinology·E J O'Keefe, W J Pledger
Jan 1, 1983·Molecular and Cellular Biology·C D ScherK L Locatell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.