The tumor suppressor gene HIC1 (hypermethylated in cancer 1) is a sequence-specific transcriptional repressor: definition of its consensus binding sequence and analysis of its DNA binding and repressive properties

The Journal of Biological Chemistry
Sébastien PinteDominique Leprince

Abstract

HIC1 (hypermethylated in cancer 1) is a tumor suppressor gene located at chromosome 17p13.3, a region frequently hypermethylated or deleted in human tumors and in a contiguous-gene syndrome, the Miller-Dieker syndrome. HIC1 is a transcriptional repressor containing five Krüppel-like C(2)H(2) zinc fingers and an N-terminal dimerization and autonomous repression domain called BTB/POZ. Although some of the HIC1 transcriptional repression mechanisms have been recently deciphered, target genes are still to be discovered. In this study, we determined the consensus binding sequence for HIC1 and investigated its DNA binding properties. Using a selection and amplification of binding sites technique, we identified the sequence 5'-(C)/(G)NG(C)/(G)GGGCA(C)/(A) CC-3' as an optimal binding site. In silico and functional analyses fully validated this consensus and highlighted a GGCA core motif bound by zinc fingers 3 and 4. The BTB/POZ domain inhibits the binding of HIC1 to a single site but mediates cooperative binding to a probe containing five concatemerized binding sites, a property shared by other BTB/POZ proteins. Finally, full-length HIC1 proteins transiently expressed in RK13 cells and more importantly, endogenous HIC1 proteins from t...Continue Reading

References

Oct 14, 1994·Biochemical and Biophysical Research Communications·N KawamataN Aoki
Jul 15, 1994·Genes & Development·V J Bardwell, R Treisman
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y Choo, A Klug
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·P DhordainD Leprince
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·K F AhmadG G Privé
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·S DeltourD Leprince
Jun 3, 2000·Nature Genetics·M BarnaP P Pandolfi
Mar 21, 2001·Human Molecular Genetics·S B BaylinJ G Herman
Mar 29, 2001·Genes & Development·A Wynshaw-Boris, M J Gambello
May 22, 2001·Cellular and Molecular Life Sciences : CMLS·S Iuchi
Feb 28, 2002·Molecular and Cellular Biology·Ari MelnickJonathan D Licht
Apr 3, 2002·The EMBO Journal·Tokameh MahmoudiC Peter Verrijzer
Jun 8, 2002·Molecular and Cellular Biology·Sophie DeltourDominique Leprince
Oct 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xing WangB Hilda Ye
Nov 1, 2002·Developmental Cell·Maria BarnaPier Paolo Pandolfi
May 17, 2003·The Journal of Biological Chemistry·Frank Pessler, Nouria Hernandez
Jun 13, 2003·Oncogene·Sarah IvinsArthur Zelent
Aug 9, 2003·American Journal of Human Genetics·Jessica YinglingAnthony Wynshaw-Boris
Dec 24, 2003·Molecular Cell·K Farid AhmadGilbert G Privé
Dec 31, 2003·Trends in Genetics : TIG·Michael Lehmann
Apr 20, 2004·Biochimica Et Biophysica Acta·Stéphanie BertrandDominique Leprince

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Citations

Mar 17, 2006·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Shih-Hwa ChiouTai-Tong Wong
Sep 20, 2005·Journal of Cancer Research and Clinical Oncology·Levent B BederKenji Shimizu
Apr 25, 2012·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Jianghua ZhengJianhua Wang
Nov 19, 2005·Nature Reviews. Cancer·Frans M van Roy, Pierre D McCrea
Jun 16, 2009·The Journal of Biological Chemistry·Capucine Van RechemDominique Leprince
Nov 8, 2008·Journal of Pediatric Hematology/oncology·David R CzuchlewskiQian-Yun Zhang
Mar 19, 2008·Genes & Development·Kimberly J BriggsD Neil Watkins
Sep 18, 2004·European Journal of Biochemistry·Tony LefebvreDominique Leprince
Oct 7, 2005·Genome Biology·Peter J StogiosGilbert G Privé
Jan 10, 2006·Neurosurgical Focus·Janet C LindseySteven C Clifford
Feb 19, 2013·The Journal of Biological Chemistry·Vanessa DehennautDominique Leprince
Apr 10, 2013·Expert Opinion on Therapeutic Targets·Brian R Rood, Dominique Leprince
Jun 3, 2009·Biochemical and Biophysical Research Communications·Capucine Van RechemDominique Leprince
Aug 30, 2008·The International Journal of Biochemistry & Cell Biology·Capucine FleurielDominique Leprince
Mar 6, 2008·British Journal of Haematology·Christian BritschgiMario P Tschan
Feb 6, 2007·International Journal of Cancer. Journal International Du Cancer·Christian N ArnoldHubert E Blum
Sep 26, 2006·Trends in Cell Biology·Kevin F Kelly, Juliet M Daniel
Nov 28, 2012·Biochemical and Biophysical Research Communications·Vanessa DehennautDominique Leprince
Oct 1, 2013·Biochemical and Biophysical Research Communications·Marion DubuissezDominique Leprince
Dec 29, 2011·Progress in Neuro-psychopharmacology & Biological Psychiatry·Rudi HwangJames L Kennedy
Sep 27, 2013·Cell Cycle·Ying-Mei LinHsiu-Ming Shih
Jun 8, 2014·The International Journal of Biochemistry & Cell Biology·Anjan Kumar PradhanSoumen Chakraborty
Apr 19, 2012·Biochemical and Biophysical Research Communications·Vanessa DehennautDominique Leprince
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Guangcun ChengXianqun Fan
Oct 22, 2016·Biochimica Et Biophysica Acta·Sheng ZengYong Xu
Dec 24, 2016·Cell Death and Differentiation·Yanbo WangXi Chen
Oct 12, 2016·Scientific Reports·Valentin Le DouceChristian Schwartz
May 4, 2017·The Journal of Pathology·Mingang HaoJianhua Wang
Jan 26, 2018·Oncogene·Anette SzczepnyD Neil Watkins
Sep 12, 2018·The Journal of Clinical Investigation·Yingying WangJianhua Wang
Jun 10, 2006·The FEBS Journal·Nicolas Stankovic-ValentinDominique Leprince
May 28, 2019·FEBS Letters·Ji-Yang SongMan-Wook Hur

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