The tumor suppressor gene product APC is hyperphosphorylated during the M phase

Biochemical and Biophysical Research Communications
R N BhattacharjeeT Akiyama

Abstract

The APC gene is mutated in familial adenomatous polyposis and sporadic colorectal tumors. The product of this gene is a 300 kDa cytoplasmic protein and its overexpression results in the block of cell cycle progression from the G0/G1 to the S phase. In the present study, we studied the expression and phosphorylation of the APC protein through the cell cycle. The APC protein was found to be constantly expressed and phosphorylated at serine and threonine residues. Moreover, the APC protein immunoprecipitated from cells arrested in the M phase by nocodazole treatment migrated in SDS-PAGE more slowly than those from the G1 and S phases. Phosphatase treatment abolished this M phase-specific retarded migration, suggesting that APC is transiently hyperphosphorylated in the M phase.

Citations

Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Miwako Kato HommaYoshimi Homma
Jun 19, 2007·Medical Molecular Morphology·Takao SendaAtsushi Shimomura
Oct 17, 1998·The Journal of Biological Chemistry·A BauerJ Stappert
Apr 28, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·K H Goss, J Groden
Jul 17, 2021·Oncology Reviews·Olivia NoeJohn Nemunaitis

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