The tumor suppressor Rb and its related Rbl2 genes are regulated by Utx histone demethylase

Biochemical and Biophysical Research Communications
Minoru TerashimaTakeshi Suzuki

Abstract

Utx is a candidate tumor suppressor gene that encodes histone H3 lysine 27 (H3K27) demethylase. In this study, we found that ectopic expression of Utx enhanced the expression of retinoblastoma tumor suppressor gene Rb and its related gene Rbl2. This activation was dependent on the demethylase activity of Utx, and was suggested to contribute to the decreased cell proliferation induced by Utx. A chromatin immunoprecipitation assay showed that over-expressed Utx was associated with the promoter regions of Rb and Rbl2 resulting in the removal of repressive H3K27 tri-methylation and the increase in active H3K4 tri-methylation. Furthermore, siRNA-mediated knockdown of Utx revealed the recruitment of endogenous Utx protein on the promoters of Rb and Rbl2 genes. These results indicate that Rb and Rbl2 are downstream target genes of Utx and may play important roles in Utx-mediated cell growth control.

References

Aug 20, 2002·Nature Genetics·Takeshi SuzukiNeal G Copeland
Oct 11, 2002·Nature·Sooryanarayana VaramballyArul M Chinnaiyan
Aug 29, 2006·Oncogene·W Du, J Pogoriler
Dec 21, 2006·Molecular and Cellular Biology·Irina IssaevaEli Canaani
Mar 8, 2007·Nature Reviews. Molecular Cell Biology·Robert J Klose, Yi Zhang
Nov 7, 2007·Nature Structural & Molecular Biology·Sukesh R BhaumikAli Shilatifard
Nov 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sunhwa HongKai Ge
Jan 30, 2008·Cell Structure and Function·Hiroshi KimuraNaohito Nozaki
Feb 25, 2009·Nucleic Acids Research·Katsuya TsuchiharaSumio Sugano
Mar 31, 2009·Nature Genetics·Gijs van HaaftenP Andrew Futreal
Sep 8, 2009·Biochemical and Biophysical Research Communications·Akihiko IshimuraTakeshi Suzuki
Feb 4, 2010·Genes & Development·Jordon K WangHoward Y Chang
Apr 9, 2010·Annual Review of Biochemistry·Nima Mosammaparast, Yang Shi

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Citations

May 1, 2013·Genes & Development·Julie-Aurore Losman, William G Kaelin
Mar 26, 2011·The Journal of Clinical Investigation·Lukas D WartmanTimothy J Ley
May 10, 2013·PLoS Genetics·Joel B BerletchChristine M Disteche
Apr 9, 2013·Cancer Science·Takeshi SuzukiAkihiko Ishimura
Feb 25, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Joni Van der MeulenPieter Van Vlierberghe
Apr 24, 2016·Urologic Oncology·Jiajun WangJianming Guo
Jun 14, 2016·TheScientificWorldJournal·Jihua RanHuiwu Li
Jan 1, 2012·Pharmaceuticals·Fernando Lizcano, Jeison Garcia
Jul 25, 2019·Applied Immunohistochemistry & Molecular Morphology : AIMM·Gi Jeong KimYoung Hwan Kim
Aug 9, 2013·Human & Experimental Toxicology·X GuoZ Cai
Sep 17, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael L NickersonDan Theodorescu

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